GeneChip® Two-Cycle Target Labeling and Control Reagents
The Two-Cycle Target Labeling and Control Reagent package provides essential labeling and control reagents with one convenient part number for target preparation from 10 - 100 ng of total RNA. This protocol is robust, streamlined, and optimized for the GeneChip platform to provide a high success rate when using limited starting material.
As part of the completely integrated GeneChip reagent product line for expression analysis, the GeneChip Two-Cycle cDNA Synthesis Kit offers:
•
Specific applications
in analyzing limited starting materials, such as small biopsies, samples
obtained from laser capture microdissection, or flow cytometry
•
Optimal results based on proven, robust, and streamlined
protocol that completes target labeling in only 2 1/2 days • No cDNA cleanup step in the 1st
cycle • No precipitation or speed vacuum • Increased volume for 1st
cycle cDNA synthesis for improved ease-of-use • IVT Labeling performed overnight
•
Consistent
performance with SuperScript™ II from Invitrogen for reverse transcription
•
Limited license
to Van Gelder/Eberwine patents via Incyte
Individual reagents can also be ordered seperately.
Note: MEGAscript® T7 Kit needs to be ordered directly from Ambion for the intermediate IVT Step with unlabeled Nucleotides.
View flowchart of Two-Cycle Target Labeling Assay.
