Affymetrix - GeneChip® HT One-Cycle cDNA Synthesis Kit (Manufactured by Invitrogen for Affymetrix)

Description

GeneChip® HT One-Cycle cDNA Synthesis Kit (Can also be ordered as part of the HT One-Cycle Target Labeling and Controls Kit)

sufficient for 96 reactions


GeneChip® HT One-Cycle cDNA Synthesis Kit (Manufactured by Invitrogen for Affymetrix)

The GeneChip® HT One-Cycle cDNA Synthesis Kit is uniquely configured and tested for GeneChip target labeling using the GeneChip® Array Station. It provides all necessary reagents in one convenient kit for synthesis of double-stranded cDNA containing the T7 promoter sequence from total RNA.

The resultant cDNA is then purified and serves as a template in the subsequent in vitro transcription (IVT) reaction for complementary RNA (cRNA) amplification and biotin labeling to hybridize to GeneChip® brand arrays.

As part of the completely integrated GeneChip reagent product line for expression analysis using the GeneChip Array Station, the GeneChip HT One-Cycle cDNA Synthesis Kit offers:

•	Streamlined workflow and 96-reaction packaging, well suited for large-scale studies enabled by the GeneChip Array Station

•	Optimal results based on a proven, robust protocol

•	Consistent performance with SuperScript™ II from Invitrogen for reverse transcription

•	Limited license to Van Gelder/Eberwine patents via Incyte

Kit Components

Component name	Quantity	Volume
T7-Oligo(dT) Primer, 50 µM	1 tube	130 µL
5X 1st Strand Reaction Mix	1 tube	460 µL
DTT, 0.1M	1 tube	230 µL
dNTP, 10 mM	1 tube	460 µL
SuperScript™ II	1 tube	120 µL
5X 2nd Strand Reaction Mix	1 tube	3,300 µL
E.coli DNA Ligase	1 tube	110 µL
E.coli DNA Polymerase I	1 tube	440 µL
RNase H	1 tube	110 µL
T4 DNA Polymerase	1 tube	280 µL
5X T4 DNA Polymerase Buffer	1 tube	60 µL

The HT One-Cycle cDNA Synthesis Kit is also available as part of the complete GeneChip® HT One-Cycle Target Labeling and Controls Kit.

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