The GeneChip® HT IVT Labeling Kit was designed for in vitro transcription (IVT), amplification, and biotin-labeling for use with the GeneChip® Array Station which automates target preparation for GeneChip® brand arrays.
Double-stranded cDNA containing the T7 promoter sequence is used as a template, generated by using the GeneChip® HT One-Cycle cDNA Synthesis Kit.
As part of the completely integrated GeneChip reagent product line for expression analysis, the GeneChip HT IVT Labeling Kit offers:
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Consistent yield and incorporation efficiency
Utilizes a proprietary synthetic biotinylated nucleotide analog in the IVT reaction with MEGAscript® reagents from Ambion to obtain high cRNA yield and uniform incorporation efficiency. |
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Quality tested and standardized system for excellent performance on GeneChip arrays
Designed specifically for the GeneChip Array Station and quality controlled as a complete system with the other accompanying reagents from Affymetrix. |
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Streamlined experimental workflow
The larger 96-reaction kit size is well suited for the complex, large-scale studies enabled by the GeneChip Array Station. |
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| 10X IVT Labeling Buffer |
1 tube |
675 µL |
| IVT Labeling Enzyme Mix |
1 tube |
675 µL |
| IVT Labeling NTP Mix |
2 tubes |
1,010 µL |
| 3'-Labeling Control (0.5 µg/µL) |
1 tube |
10 µL |
| T7 RNA Polymerase |
1 tube |
115 µL |
| 5X Fragmentation |
1 tube |
855 µL |
The HT IVT Labeling Kit is also available as part of the complete GeneChip® HT One-Cycle Target Labeling and Controls Kit. |
