Standardized, reliable assays and reagents are integral components
of the GeneChip® system solution, allowing researchers to obtain
high-quality data and reproducible results on GeneChip brand arrays.
These robust products are convenient and easy to use for simplified
sample and target preparation.
Optimized assays are specifically designed for a wide range
of sample types and applications.
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Labeling assays uniquely complement Affymetrix’ sequence
and probe selection strategies for whole-genome expression analysis
with optimal performance.
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Several target labeling and sample preparation assays are
available, particularly suited for working with diverse starting
sample types, providing ease of use and
confidence for GeneChip expression analysis.
Validated reagents are quality tested on
GeneChip® expression
arrays.
•
All reagents are assured for success and consistency on
GeneChip arrays.
•
All arrays include built-in controls. The
corresponding set of control reagents is available to monitor
the entire GeneChip expression experiment, from target
preparation to array hybridization.
Standardized single-color labeling for ultimate comparability.
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Single-color labeling facilitates in silico comparison of
expression data across any number of arrays, experiments,
and labs. Researchers can now compare, publish, and share
data without limitation.
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Tailored assays for standard or limited starting materials
— Two eukaryotic GeneChip® Target Labeling Assay protocols
are available to ensure successful expression analysis. Scientists
can select the procedure that best matches their sample requirements.
•
Linear amplification and labeling strategy
for reliable results — Both assays utilize an Oligo(dT)-primed,
in vitro transcription-based linear amplification strategy to
generate high-yield, biotinylated targets from the 3'-end. This
ensures compatibility with the current array design strategy,
which targets sequences in the
region of 600 bases proximal to the 3'-end of each transcript.
One-Cycle and Two-Cycle Target Labeling Assays were extensively
tested on GeneChip arrays to reliably detect transcript expression
levels.
