The GeneChip® Rat Genome 230 2.0 Array is a powerful tool for toxicology, neurobiology, and other applications using rat as a model organism.
- Provides comprehensive coverage of the transcribed rat genome on a single
array
- Comprised of more than 31,000 probe sets, analyzing over 30,000 transcripts
and variants from over 28,000 well-substantiated rat genes
- The publicly available draft of the rat genome and leading public rat databases
were used to refine sequences and provide a higher quality of data output
- Sophisticated bioinformatics tools available through the NetAffx™ Analysis
Center to rapidly obtain biologically meaningful results
Array Profile
All probe sets represented on the GeneChip Rat Expression Set 230 are included
on the GeneChip Rat Genome 230 2.0 Array. Sequences used in the design of the
GeneChip Rat Genome 230 2.0 Array were selected from GenBank®, dbEST, and
RefSeq. The sequence clusters were created from the UniGene database (Build
99, June 2002) and then refined by analysis and comparison with the publicly
available draft assembly of the rat genome from the Baylor College of Medicine
Human Genome Sequencing Center (June 2002).
The GeneChip Rat Genome 230 2.0 Array includes representation of the RefSeq
database sequences and probe sets related to sequences and refined EST clusters
previously represented on the GeneChip Rat Genome U34 Set.
Oligonucleotide probes complementary to each corresponding sequence are synthesized
in situ on the arrays. Eleven pairs of oligonucleotide probes are used to measure
the level of transcription of each sequence represented on the GeneChip Rat
Genome 230 2.0 Array.
Instrument Software Requirements
- GeneChip® Scanner 3000, enabled for High-Resolution Scanning*
- GeneChip® Operating Software (GCOS) v1.1.1, which contains the High-Resolution
Scanning Update
* GeneChip Scanner 3000 High-Resolution Update is standard on all instruments
shipped starting in September 2003 with serial number series 502. Previous versions
(serial number series 501) will require the 00-0110 GeneChip Scanner 3000 High-Resolution
Update to be installed. |
