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GeneChip® Human Tiling 2.0R Array Set

Most comprehensive whole-genome array set for studying protein/DNA interactions in chromatin immunoprecipitation (ChIP) experiments.

High-density 35 base pair probe spacing offers a more accurate view of protein/DNA interactions
25-mer probes yield the most specific hybridization results available

Array Profile

The GeneChip® Human Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments. This set of seven arrays contains approximately 45 million oligonucleotide probes to interrogate the human genome. Each array in the set contains over 6.5 million perfect match probes to specifically interrogate genomic regions.

Sequences used in the design of the Human Tiling 2.0R Array Set were selected from NCBI human genome assembly (Build 34). Repetitive elements were removed by RepeatMasker. The Human Tiling 2.0R Array Set is available either as a whole-genome set of seven arrays (P/N 900772), or individual arrays from the set may be purchased separately. The GeneChip® Human Promoter 1.0R Array is a subset of the Human Tiling 2.0R Tiling Array Set, and the content was chosen specifically to analyze protein/DNA interactions within promoter regions.

Probes are tiled at an average resolution of 35 base pair, as measured from the central position of adjacent 25-mer oligos, leaving a gap of approximately 10bp between probes. Oligonucleotide probes are synthesized in situ complementary to each corresponding sequence.

Instrument and Software Requirements

GeneChip® Scanner 3000 7G
GeneChip® Operating Software (GCOS) version 1.3 or higher

Visit the Affymetrix ChIP-on-Chip Web Forum and share protocols, experiences, hints and tips on best practices for ChIP-on-chip and MeDIP-on-chip applications. Free registration is required to view or create posts.

ORDERING INFORMATION

Part # Description
900772 GeneChip® Human Tiling 2.0R Array Set
Complete A-G genome set
1 7-Array set
900779 GeneChip® Human Tiling 2.0R A Array
Covers chromosomes 1, 6
6 Arrays
900780 GeneChip® Human Tiling 2.0R B Array
Covers chromosomes 2, 9, 19
6 Arrays
900781 GeneChip® Human Tiling 2.0R C Array
Covers chromosomes 3, 21, 22, X, Y, mitochondria
6 Arrays
900782 GeneChip® Human Tiling 2.0R D Array
Covers chromosomes 4, 15, 18, 20
6 Arrays
900783 GeneChip® Human Tiling 2.0R E Array
Covers chromosomes 5, 7, 16
6 Arrays
900784 GeneChip® Human Tiling 2.0R F Array
Covers chromosomes 8, 11, 12
6 Arrays
900785 GeneChip® Human Tiling 2.0R G Array
Covers chromosomes 10, 13, 14, 17
6 Arrays
 
How to order products from Affymetrix.
 
Pricing Information
RELATED PRODUCTS
Control Oligo B2, 3nM
GeneChip® WT Double-Stranded DNA Terminal Labeling Kit
Affymetrix® Tiling Analysis Software
Integrated Genome Browser (IGB)
SUPPORT
ChIP-on-chip (pdf, 487 KB)
Human Tiling Arrays (pdf, 260 KB)
Human Tiling 2.0R Array Set (zip, 1 GB)
View More...
Chromatin Immunoprecipitation Assay Protocol (pdf, 679 KB)
Human Tiling 2.0R Array Set (pdf, 77 KB)
Tiling Analysis Software Tutorial
ADDITIONAL SUPPORT
Fluidics Scripts
Scientific Forum, 1st European ChIP-on-chip
Webinar, Human Tiling Array - September 15, 2005
Webinar, Six-part ChIP-on-chip Symposia Series
All Support Materials for Human Tiling 2.0R Array Set
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