Performance

The Human Gene 1.0 ST Array has shown comparable gene-level performance to classical 3’-based expression arrays, such as the Human Genome U133 Plus 2.0 and Exon 1.0 ST Arrays for genes common to all three platforms (see figure ). Heart and brain tissue mixtures studies, as well as a Latin Square spike-in experiments, have demonstrated high sensitivity and reproducibility. For more detailed information on these experiments please refer to the Human Gene 1.0 ST Array Performance white paper.

The Human Gene 1.0 Array has comparable performance to the HG-U133 and Exon 1.0 ST Array as demonstrated by Latin Square spike-in experiments. For each array, multiple comparisons were done for different concentrations (as shown along the x-axis). A t-statistic was computed between the three chips with no spike and the three chips with a concentration listed on the x-axis. From this the percentage of detected spikes (at an empirical false positive rate of 0.01) was determined (as shown along the y-axis). See the Human Gene 1.0 ST Array Performance white paper for more details.