Comprehensive Coverage of the Entire Gene Locus

The Human Gene 1.0 ST Array interrogates 28,869 well-annotated genes with 764,885 distinct probes. The design of the Human Gene 1.0 ST Array was based on the March 2006 (UCSC hg18, NCBI Build 36) human genome sequence assembly with comprehensive coverage of RefSeq, Ensembl and putative complete CDS GenBank transcripts. The Human Gene 1.0 ST Array has greater than 99 percent coverage of sequences present in the RefSeq database.

The Gene 1.0 ST Array is a perfect match (PM)-only design with probes that hybridize to sense target. Background is estimated using a set of approximately 20,000 generic background probes. Standard poly-A controls and hybridization controls are also represented on the array to allow convenient troubleshooting along the entire experimental process.

The Gene 1.0 ST Array uses a subset of probes from the Exon 1.0 ST Array and covers only well-annotated content. Like the Exon 1.0 ST Array, “gene-level” analysis of multiple probes on different exons are summarized into accumulative expression value representing all transcripts from the same gene.