The Whole Transcript (WT) Sense Target Labeling Assay Has an Updated Protocol
The GeneChip® Human Gene 1.0 ST Array is the latest product in the family of Affymetrix expression arrays offering whole-transcript coverage. Each of the 28,869 genes is represented on the array by approximately 26 probes spread across the full length of the gene, providing a more complete and more accurate picture of gene expression than 3’ based expression array designs. The small format of the array makes it a cost-effective expression profiling solution for new microarray users.
The Gene 1.0 ST Array is part of a complete solution for gene expression analysis that includes Whole Transcript (WT) Sense Target Labeling and Control Reagents, fluidics and scanning instrumentation and basic analysis software. The array contains the most up-to-date content of well-annotated genes, and data is analyzed using a simple workflow.
The Gene 1.0 ST Array uses a subset of probes from the Human Exon 1.0 ST Array and covers only well-annotated content. Like the Exon 1.0 ST Array, “gene-level” analysis of multiple probes on different exons is summarized into an expression value representing all transcripts from the same gene.
More Information:
Learn more about Human Gene 1.0 ST Array coverage
Learn more about Human Gene 1.0 ST Array performance
Details about the complete system for expression analysis with the Gene 1.0 ST Array
|
Array Format |
169 |
| Feature Size |
5 µm |
| Total Number of Distinct Probes |
764,885 |
| Oligonucleotide Probe Length |
25-mer probes |
| Required Orientation of Labeled Targets to be Hybridized to the Array |
Sense |
| Gene-level Probe Sets with Ensembl Support |
28,132 |
| Gene-level Probe Sets with Putative Full-length Transcript Support (GenBank and RefSeq) |
19,734 |
|
