The GeneChip® HT Mouse Genome 430 Array Plate Set enables researchers to perform large-scale studies by analyzing whole-genome expression for as many as 96 mouse samples at a time. The set comprises a 430A and 430B plate, measuring expression levels for more than 34,000 well-characterized genes and UniGene clusters.
Researchers can process up to 96 individual A or B arrays per plate; each array contains the same probe sets as those represented on the MG-430A and MG-430B Array cartridge formats.
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Measure gene expression of more than 39,000 transcripts and variants, including more than 34,000 well-characterized genes and UniGene clusters per sample |
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Get accurate and reproducible data by using multiple independent measurements for each transcript, with more than 45,000 probe sets and 1 million distinct oligonucleotide features |
Scientists are using the HT system in large-scale studies to:
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Reduce labor costs and time of analysis |
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Increase standardization within and across sites |
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Simplify management of running multiple microarrays in parallel |
Array Plate Design
The A and B plate consist of 96 appendages, to which as many as 96 individual microarrays are attached. The plates are constructed to be spatially compatible with conventional 96-well plate formats and liquid-handling equipment. This configuration is designed to address the needs of large-scale experiments by greatly simplifying the management of multiple microarrays run in parallel, reducing labor costs and enabling increased standardization within studies and across sites. For each plate, two configurations are offered:
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24-Array Plate – 24 HT MG-430A or HT MG-430B microarrays are positioned on a single plate of 24 arrays for simultaneous processing and analysis |
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96-Array Plate – 96 HT MG-430A or HT MG-430B microarrays are positioned on a single plate of 96 arrays |
Content Profile
The probe sets were selected from sequences derived from GenBank®, dbEST and RefSeq. The sequence clusters were created from the UniGene database (Build 107, June 2002) and then refined by analysis and comparison with the publicly available draft assembly of the mouse genome from the Whitehead Institute Center for Genome Research (MSCG, April 2002).
Oligonucleotide probes complementary to each corresponding sequence are synthesized in situ on the array. Eleven pairs of oligonucleotide probes, including a perfect match and mismatch probe, are used to measure the level of transcription of each sequence represented on the GeneChip® HT Mouse Genome 430 Array Plate Set.
Reagents, Instrument and Software Requirements
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