The GeneChip® HT Human Genome U133 Array Plate Set enables researchers to perform large-scale studies by analyzing whole-genome expression for up to 96 samples at a time. The set comprises a U133A and U133B plate, measuring expression levels for more than 33,000 well-characterized genes and UniGene clusters.
Researchers can process up to 96 individual A or B arrays per plate. Each array contains the same probe sets as those represented on the HG-U133A Array and HG-U133B Array cartridge formats.
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Measure gene expression of approximately 39,000 transcripts and variants, including more than 33,000 well-characterized genes and UniGene clusters per sample |
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Get accurate and reproducible data by using multiple independent measurements for each transcript, with more than 45,000 probe sets and 1 million distinct oligonucleotide features |
Scientists are using the HT system in large-scale studies to:
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Analyze up to 96 samples at a time |
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Increase standardization within and across sites |
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Simplify management of running multiple microarrays in parallel |
Array Plate Design
Each A and B plate consists of 96 appendages, to which as many as 96 individual microarrays are attached. The plates are constructed to be spatially compatible with conventional 96-well plate formats and liquid-handling equipment. This configuration is designed to address the needs of large-scale experiments by greatly simplifying the management of multiple microarrays run in parallel, reducing labor costs and enabling increased standardization within studies and across sites. For each plate, two configurations are offered:
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24-Array Plate – 24 HT HG-U133A or HT HG-U133B microarrays are positioned on a single plate of 24 arrays for simultaneous processing and analysis |
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96-Array Plate – 96 HT HG-U133A or HT HG-U133B microarrays are positioned on a single plate of 96 arrays |
Content Profile
Sequences used in the design of the array were selected from GenBank®, dbEST and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz, Golden-Path human genome database (April 2001 release).
Oligonucleotide probes complementary to each corresponding sequence are synthesized in situ on the array. Eleven pairs of oligonucleotide probes are used to measure the transcription level of each sequence represented on the GeneChip® HT Human Genome U133 Array Plate Set.
Reagents, Instrument and Software Requirements
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