Affymetrix - GeneChip® Exon Array System

GeneChip® Exon and Gene Array Systems

See the Whole Gene Expression Picture—Accurately and Precisely

Affymetrix GeneChip® Exon and Gene Arrays offer a more complete and accurate picture of overall gene expression than traditional 3'-based expression arrays, by enabling you to interrogate the entire length of the gene, not just the 3' end. Combined with the GeneChip® Whole Transcript Assay, you not only get a more accurate picture of whole-genome, whole-transcript gene expression, but you can also see precisely what is being expressed.

See the real biology
Real biology happens at the transcript level, not the gene level
Whole Transcript Expression and Alternative Splicing: Exon 1.0 ST Arrays

GeneChip Exon Arrays provide the most comprehensive coverage of the genome and enable two complementary levels of analysis-gene expression and alternative splicing. Multiple probes per exon enable “exon-level” analysis and allow you to distinguish between different isoforms of a gene. This exon-level analysis on a whole-genome scale opens the door to detecting specific alternative splicing events that may play a central role in disease mechanism and etiology. You can also perform “gene-level” analysis, in which multiple probes on different exons are summarized into a single expression value of all transcripts from the same gene, enabling similar analysis workflows to 3'-gene expression.

See the whole gene
Measure expression using the entire gene instead of just the 3'
Whole Transcript Expression: Gene 1.0 ST Arrays

The Gene 1.0 ST Array System offers a cost-effective solution for whole-genome, whole-transcript expression analysis for human, mouse and rat. Each of the well-annotated genes is represented by approximately 26 probes spread across the full length of the gene, providing a more complete and accurate picture of gene expression than 3'-based expression array designs. All 26 probes are automatically summarized into a single expression value per gene, allowing standard analysis software packages and simple analysis workflows to be used to analyze data. Gene 1.0 ST Arrays are the most advanced and cost-effective gene expression profiling option for new microarray users.

Research Profiles

Researchers at Erasmus Medical Center in the Netherlands used the GeneChip® Human Exon 1.0 ST Array to discover expression profiles with distinct splice variants that more accurately classify two forms of glial cancers. The team has also identified more than 700 novel exon-skipping events in these gliomas, allowing them to pinpoint sequence mutations in potential causative genes. Their findings may help clinicians more accurately diagnose the multiple classes and variable prognoses of brain cancer and suggest novel therapeutic targets for gliomas.

Researchers at Millennium Pharmaceuticals have confirmed specific "cassette" exons in the CD44 gene that are highly expressed in primary tumor colon cancer cell lines and not in metastatic tumor or HeLa cell lines. These findings may lead to the use of CD44 splice variants as a diagnostic or prognostic marker for colon cancer.

Data Courtesy of Millennium

WEBINARS

Demonstration of Partek Genomics Suite™ for the Analysis of GeneChip® Exon Arrays

View a presentation featuring Partek's Tom Downey

Demonstration of Biotique Systems' XRAY for the Analysis of GeneChip® Exon Arrays

View a presentation featuring Biotique Systems' John Burke

EXON AND GENE ARRAY PRODUCTS

Arrays

Assays and Reagents

Data Analysis Solutions

Instruments