GeneChip® HT Human Genome U133 Array Plate Setは、一度に最大96サンプルの全ゲノム発現解析が可能で、大規模研究を行うことができます。このセットはU133AとU133Bプレートから成り、33,000を超える既知遺伝子とUniGeneクラスターの発現レベルを測定します。
これにより、プレート当たり最大96のAまたはBアレイを処理することができます。 各アレイには、HG-U133AアレイおよびHG-U133Bアレイカートリッジフォーマットと同じプローブセットが採用されています。
- サンプル当たり33,000以上の既知遺伝子とUniGeneクラスターを含む約39,000の転写産物およびバリアントの遺伝子発現を測定します。
- 45,000を超えるプローブセット、100万のオリゴヌクレオチドによりで、各転写産物を独立した複数の測定することにより、正確で再現性の高いデータが得られます。
研究者は次の目的で大規模研究にHTシステムを採用しています:
- 最大96サンプルを一度に解析。
- 研究所内や複数の研究所間での標準化が向上。
- 複数のマイクロアレイを並行進行する管理が簡単。
アレイプレートデザイン
AおよびBプレートは最大96の個別マイクロアレイが付属しています。 このプレートは通常の96ウェルプレートフォーマットでリキットハンドリング装置が使用できる空間設計になっています。 この仕様は大規模研究のニーズに対応した設計で、複数のマイクロアレイを並行進行する際の管理を大幅に簡略化し、労力コストが削減でき、研究所内や複数の研究所間での標準化を向上させることができます。 各プレートについて、次の2つの仕様が提供されています。
- 24アレイプレート – 24HT HG-U133AまたはHT HG-U133Bマイクロアレイは、24アレイがプレート1枚に配置されており、同時処理・解析できます。
- 96アレイプレート – 96HT HG-U133AまたはHT HG-U133Bマイクロアレイは、96アレイがプレート1枚に配置されています。
コンテンツプロフィール
アレイの設計に使用されている配列は、GenBank®、dbEST、RefSeqから選択されています。 配列クラスターはUniGeneデータベース(ビルド133、2001年4月20日)から作成され、他の数多くの公開データベース(ワシントン大学ESTトレースレポジトリやカリフォルニア大学サンタクルーズ校ゴールデンパスヒューマンゲノムデータベース(2001年4月公開)など)と比較解析することによってさらに正確さを増しています。
各配列を補うオリゴヌクレオチドプローブは、アレイ上でin situ合成されます。 GeneChip® HT Human Genome U133 Array Plate Setの各配列の転写レベルの測定には、11組のオリゴヌクレオチドプローブが使用されます。

Data Sheets
HT Array Replacement Guideline (pdf, 179 KB)
Human Genome U133 Arrays (pdf, 1.76 MB)
White Papers
HT and Cartridge IVT Expression Arrays (pdf, 601 KB)
Performance Evaluation of the GeneChip® HT Array Plate Scanner (pdf, 1.96MB)
GeneChip® HT Human Genome U133 Array Plates
| Part # | Description | Includes |
|---|---|---|
| 900750 | GeneChip® HT Human Genome U133A 24-Array Plate |
Contains 4 HT HG-U133A 24-Array Plates |
| 900751 | GeneChip® HT Human Genome U133A 96-Array Plate |
Contains 1 HT HG-U133A 96-Array Plates |
| 901042 | GeneChip® HT Human Genome U133B 24-Array Plate |
Contains 4 HT HG-U133B 24-Array Plates |
| 901043 | GeneChip® HT Human Genome U133B 96-Array Plate |
Contains 1 HT HG-U133B 96-Array Plates |
| 901078 | GeneChip® HT Human Genome U133 24-Array Plate Set |
Contains 4 HT HG-U133A 24-Array Plates Contains 4 HT HG-U133B 24-Array Plates |
| 901077 | GeneChip® HT Human Genome U133 96-Array Plate Set |
Contains 1 HT HG-U133A 96-Array Plate Contains 1 HT HG-U133B 96-Array Plate |
GeneChip® HT Human Genome U133 Array Plates with Reagents
| Part # | Description | Includes |
|---|---|---|
| 900832 | GeneChip® HT Human Genome U133A 24-Array Plate with Reagents * |
Contains 4 HT HG-U133A 24-Array Plates Contains 1 HT One-Cycle Target Labeling and Control Kit, 96 Reactions |
| 900833 | GeneChip® HT Human Genome U133A 96-Array Plate with Reagents* |
Contains 1 HT HG-U133A 96-Array Plate Contains 1 HT One-Cycle Target Labeling and Control Kit, 96 Reactions |
| 901048 | GeneChip® HT Human Genome U133B 24-Array Plate with Reagents* |
Contains 4 HT HG-U133B 24-Array Plates Contains 1 HT One-Cycle Target Labeling and Control Kit, 96 Reactions |
| 901049 | GeneChip® HT Human Genome U133B 96-Array Plate with Reagents* |
Contains 1 HT HG-U133B 96-Array Plate Contains 1 HT One-Cycle Target Labeling and Control Kit, 96 Reactions |
| 901080 | GeneChip® HT Human Genome U133 24-Array Plate Set with Reagents* |
Contains 4 HT HG-U133A 24-Array Plates Contains 4 HT HG-U133B 24-Array Plates Contains 1 HT One-Cycle Target Labeling and Control Kit |
| 901079 | GeneChip® HT Human Genome U133 96-Array Plate Set with Reagents* |
Contains 1 HT HG-U133A 96-Array Plate Contains 1 HT HG-U133B 96-Array Plate Contains 1 HT One-Cycle Target Labeling and Control Kit |
| * All trays necessary for Array Plate processing are packaged with HT Array Plates listed above. |
Required Products
| Part # | Description | Includes |
|---|---|---|
| 900686 | GeneChip® HT One-Cycle Target Labeling and Controls Kit | Sufficient for 96 reactions |
| 00-0162 00-0235 |
GeneChip® Array Station | See Product Page |
| 00-0332 | GeneChip® HT Array Plate Scanner | |
| GeneChip® Operating Software | Available Via Download | |
| HT Image Reader | ||
| HT Data Transfer Tool |
Related Products
| Part # | Description | Includes |
|---|---|---|
| Assays and Reagents | See Product Page |
FAQs
Manuals
Probe Set Data in MAGE-ML Format
Library Files
HT Human Genome U133A (zip, 13 MB)
HT Human Genome U133B (zip, 13 MB)
Alignment, Annotation, and Sequence Files
Information about using Alignment, Annotation, and Sequence files
Current NetAffx Annotation Files
HT_HG-U133A Annotations, CSV format, Release 32 (11 MB, 6/9/11)
HT_HG-U133B Annotations, CSV format, Release 32 (8.1 MB, 6/9/11)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 32 (46 MB, 6/9/11)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 32 (26 MB, 6/9/11)
HT_HG-U133A BLASTP annotations, CSV Format, Release 32 (3.9 KB, 6/9/11)
HT_HG-U133B BLASTP annotations, CSV Format, Release 32 (6.0 KB, 6/9/11)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 32 (4.7 MB, 6/9/11)
Note: NetAffx annotation files are intended for use in automated analysis. Some are not compatible with all spreadsheet applications. Please use the NetAffx Analysis Center to limit download data to your probe sets of interest. Annotation CSV files for the exon arrays are split into a probe set-level annotation file and a transcript cluster-level annotation file; exon array CSV files are compatible with ExACT.
Sequence Files
HT_HG-U133B Consensus Sequences FASTA Format (10.0 MB, 3/16/07)
HT_HG-U133B Control Sequences FASTA Format (26 KB, 3/16/07)
HT_HG-U133B Probe Sequences, FASTA format (4.5 MB, 3/11/09)
HT_HG-U133A Probe Sequences, Tabular (4.1 MB, 3/11/09)
HT_HG-U133B Probe Sequences, tabular format (4.0 MB, 3/11/09)
HT_HG-U133B Target Sequences FASTA Format (4.2 MB, 3/16/07)
Archived NetAffx Annotation Files
HT_HG-U133A Annotations, CSV format, Release 21 (9.5 MB, 5/31/07)
HT_HG-U133A Annotations, CSV format, Release 22 (9.9 MB, 5/31/07)
HT_HG-U133B Annotations, CSV format, Release 22 (1.2 MB, 5/31/07)
HT_HG-U133A Annotations, CSV format, Release 23 (9.3 MB, 7/12/07)
HT_HG-U133B Annotations, CSV format, Release 23 (1.2 MB, 7/12/07)
HT_HG-U133A Annotations, CSV format, Release 24 (9.4 MB, 11/6/07)
HT_HG-U133B Annotations, CSV format, Release 24 (7.3 MB, 11/6/07)
HT_HG-U133A Annotations, CSV format, Release 25 (9.8 MB, 3/18/08)
HT_HG-U133B Annotations, CSV format, Release 25 (7.5 MB, 3/18/08)
HT_HG-U133A Annotations, CSV format, Release 26 (10 MB, 7/8/08)
HT_HG-U133B Annotations, CSV format, Release 26 (7.7 MB, 7/8/08)
HT_HG-U133A Annotations, CSV format, Release 27 (10 MB, 11/30/08)
HT_HG-U133B Annotations, CSV format, Release 27 (7.7 MB, 11/30/08)
HT_HG-U133A Annotations, CSV format, Release 28 (11 MB, 3/12/09)
HT_HG-U133B Annotations, CSV format, Release 28 (7.9 MB, 3/12/09)
HT_HG-U133A Annotations, CSV format, Release 29 (11 MB, 7/1/09)
HT_HG-U133B Annotations, CSV format, Release 29 (7.9 MB, 7/1/09)
HT_HG-U133A Annotations, CSV format, Release 30 (12 MB, 11/15/09)
HT_HG-U133B Annotations, CSV format, Release 30 (8.2 MB, 11/15/09)
HT_HG-U133A Annotations, CSV format, Release 31 (11 MB, 8/23/10)
HT_HG-U133B Annotations, CSV format, Release 31 (7.9 MB, 8/23/10)
HT_HG-U133A Annotations, MAGE-ML XML format, Release 21 (45 MB, 6/15/07)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 22 (41 MB, 6/15/07)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 23 (40 MB, 7/12/07)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 24 (40 MB, 11/6/07)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 24 (25 MB, 11/6/07)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 25 (41 MB, 3/18/08)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 25 (25 MB, 3/18/08)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 26 (42 MB, 7/8/08)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 26 (25 MB, 7/8/08)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 27 (43 MB, 11/24/08)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 27 (25 MB, 11/24/08)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 28 (45 MB, 3/12/09)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 28 (26 MB, 3/12/09)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 29 (45 MB, 7/1/09)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 29 (26 MB, 7/1/09)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 30 (46 MB, 11/15/09)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 30 (26 MB, 11/15/09)
HT_HG-U133A Annotations, MAGE-ML XML Format, Release 31 (46 MB, 8/23/10)
HT_HG-U133B Annotations, MAGE-ML XML Format, Release 31 (26 MB, 8/23/10)
HT_HG-U133A BLASTP annotations, CSV format, Release 21 (2.9 MB, 11/15/06)
HT_HG-U133A BLASTP annotations, CSV Format, Release 22 (44 KB, 3/9/07)
HT_HG-U133A BLASTP annotations, CSV Format, Release 23 (40 KB, 7/12/07)
HT_HG-U133A BLASTP annotations, CSV Format, Release 24 (2.1 KB, 11/6/07)
HT_HG-U133B BLASTP annotations, CSV Format, Release 24 (4.8 KB, 11/6/07)
HT_HG-U133A BLASTP annotations, CSV Format, Release 25 (20 KB, 3/18/08)
HT_HG-U133B BLASTP annotations, CSV Format, Release 25 (37 KB, 3/18/08)
HT_HG-U133A BLASTP annotations, CSV Format, Release 26 (5.5 KB, 7/8/08)
HT_HG-U133B BLASTP annotations, CSV Format, Release 26 (4.3 KB, 7/8/08)
HT_HG-U133A BLASTP annotations, CSV Format, Release 27 (26 KB, 11/24/08)
HT_HG-U133B BLASTP annotations, CSV Format, Release 27 (39 KB, 11/24/08)
HT_HG-U133A BLASTP annotations, CSV Format, Release 28 (2.9 KB, 3/4/09)
HT_HG-U133B BLASTP annotations, CSV Format, Release 28 (4.6 KB, 3/4/09)
HT_HG-U133A BLASTP annotations, CSV Format, Release 29 (3.0 KB, 7/1/09)
HT_HG-U133B BLASTP annotations, CSV Format, Release 29 (3.0 KB, 7/1/09)
HT_HG-U133A BLASTP annotations, CSV Format, Release 30 (14 KB, 11/16/09)
HT_HG-U133B BLASTP annotations, CSV Format, Release 30 (26 KB, 11/16/09)
HT_HG-U133A BLASTP annotations, CSV Format, Release 31 (18 KB, 8/11/10)
HT_HG-U133B BLASTP annotations, CSV Format, Release 31 (32 KB, 8/11/10)
HT_HG-U133A Orthologs/Homologs, CSV format, Release 21 (3.9 MB, 11/30/06)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 22 (4.3 MB, 3/9/07)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 23 (4.2 MB, 7/12/07)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 24 (4.0 MB, 11/6/07)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 25 (4.0 MB, 3/18/08)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 26 (4.3 MB, 7/8/08)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 27 (4.6 MB, 11/24/08)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 28 (4.9 MB, 3/12/09)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 29 (4.8 MB, 7/1/09)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 30 (4.7 MB, 11/15/09)
HT_HG-U133A Orthologs/Homologs, CSV Format, Release 31 (4.7 MB, 8/11/10)
Additional Support



