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GeneChip^® Human Exon 1.0 ST Array

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2009年8月より、新しいWTアッセイが始まりました>>

GeneChip® Human Exon 1.0 ST Arrayは1エクソンあたり約4個、1遺伝子あたり約40個のプローブを備えており、遺伝子発現と選択的スプライシングという2つの相補的なレベルの解析を可能にします。

1つのエクソンに複数のプローブが対応しているため、「エクソンレベル」の解析が可能であり、1つの遺伝子の異なるアイソフォームを区別できます。このような全ゲノム規模のエクソンレベル解析は、疾患のメカニズムや病因に中心的な役割を果たしている可能性のある、使用されるエクソンの特異的な変化の検出を可能にします。

もう1つのレベルの解析は、異なるエクソンに対応する複数のプローブのデータが同一の遺伝子からの全転写産物に対する発現値へとまとめられる「遺伝子レベル」の発現解析です。

エクソンアレイは、実験的に確認されている転写配列と予測される転写配列を含む極めて包括的なゲノムのカバーを提供するものであり、これまで未確認であった新たな現象の発見をも可能とします。

この統合的なソリューションの特徴としては、以下のようなものが挙げられます：

全ゲノム中の既知および予測上の転写領域内のエクソンクラスターを100万個以上調べることが可能な、極めて高分解能の発現解析。詳しくはこちら
ランダムプライミング法を利用して転写産物の全長にわたってセンス鎖ターゲットを生成する、専用のGeneChip® Whole Transcript（WT）アッセイと試薬類。詳しくはこちら
ユーザーが決めた様々なワークフローでデータを調査、分析する機会を提供する、柔軟なデータ解析ソリューションと包括的なアノテーション。

Learn More

Human Exon 1.0 ST Array デザインサマリー
 Human Exon 1.0 ST Array Starter Pack

Read About

GeneChip® Exon Array Design
Technical Note, Array Design for the Human Exon 1.0 ST Array (pdf, 348 KB)
GeneChip® Exon Array Sample Data
Technical Note, Identifying and Validating Alternative Splicing Events

New XRAY demos for exon expression analysis

View a two-minute demon on a typical workflow using Biotique's XRAY for the analysis of GeneChip® Exon Arrays
Windows Media Player | Quicktime

View a two-minute demo on the interpretation of GeneChip®; Exon Array data from Biotique's XRAY
Windows Media Player | Quicktime

Data Sheets

Exon Array System (pdf, 3.22MB)-Japanese Version

Exon Array System (pdf, 416 KB)

Technical Notes

Array Design for the Human Exon 1.0 ST Array (pdf, 348 KB)

Guide to Probe Logarithmic Intensity Error (PLIER) Estimation (pdf, 294 KB)

Application Notes

Whole-transcript Expression Analysis (pdf, 557 kb)

White Papers

Alternative Transcript Analysis Methods for Exon Arrays v1.1 (pdf, 79 KB)

Exon Array Background Correction v1.0 (pdf, 78 KB)

Exon Probeset Annotations and Transcript Cluster Groupings v1.0 (pdf, 87 KB)

Exon Related Changes FAQ (pdf, 268 KB)

Gene Signal Estimates from Exon Arrays v1.0 (pdf, 428 KB)

Quality Assessment of Exon and Gene 1.0 ST Arrays (pdf, 180 KB)

Publications

Publications and Reference for Affymetrix® GeneChip® Exon and Gene 1.0 ST Arrays (pdf, 289 KB)

Part #	Description	Includes
900649	GeneChip® Human Exon 1.0 ST Array	Contains 2 arrays
900650	GeneChip® Human Exon 1.0 ST Array	Contains 6 arrays
900651	GeneChip® Human Exon 1.0 ST Array	Contains 30 arrays

Related Products

Part #	Description	Includes
	Affymetrix® GeneChip® Command Console® Software	Available Via Download
	Affymetrix® Expression Console™ Software	Available Via Download
	Ambion® WT Expression Kit
900720	GeneChip® Hybridization, Wash, and Stain Kit	Sufficient for 30 reactions
00-0211	GeneChip® Scanner 3000 7G with Workstation
00-0079	GeneChip® Fluidics Station 450

FAQs

Exon Array Analysis

Exon Array Design

Manuals

Ambion® WT Expression Kit (pdf, 1.14 MB)

Expression Wash, Stain and Scan User Manual for Cartridge Arrays (pdf, 5.41 MB)

WT Terminal Labeling and Hybridization for use with the Ambion® WT Expression Kit (pdf, 447 KB)

Quick Reference Cards

QC Metrics for Exon and Gene Design Expression Arrays (pdf, 123 KB)

Sample Data

Exon 1.0 ST Array Dataset

Array Comparisons

Comparison Spreadsheets User's Guide (pdf, 82 KB)

HG-U133 Plus to Human Exon 1.0 ST, Best Match (zip, 899 KB)

HG-U133 Plus to Human Exon 1.0 ST, Complex Match (zip, 36 MB)

HG-U133 Plus to Human Exon 1.0 ST, No Match (zip, 1.5 KB)

Human Exon 1.0 ST to Human Gene 1.0 ST, Best Match (zip, 485 KB)

Human Exon 1.0 ST to Human Gene 1.0 ST, Complex Match (zip, 29 MB)

Human Exon 1.0 ST to Human Gene 1.0 ST, Good Match (zip, 521 KB)

Human Exon 1.0 ST to Human Gene 1.0 ST, No Match (zip 500 B)

Library Files

Human Exon 1.0 ST Array Analysis (zip, 131 MB)

Human Exon 1.0 ST Array Probeset, and Meta Probeset Files, core, full, extended and comprehensive hg18 (zip, 13 MB)

Human Exon 1.0 ST Array, GCOS (zip, 2.2 MB)

Unsupported Human Exon 1.0 ST Array CDF Files (zip, 205 MB)

Package Inserts

Human Exon 1.0 ST Array (pdf, 522 KB)

Alignment, Annotation, and Sequence Files

Information about using Alignment, Annotation, and Sequence files

IGB Annotation Files

Annotations, Hg16/Build 34, BGN

Annotations, Hg17/Build 35, BGN

Annotations, Hg18/Build 36, BGN

Note: The IGB BGN annotation files can be used to load up transcript cluster, probeset, and probe level annotations for the Affymetrix GeneChip® Arrays into IGB. These files allow the user to quickly load a whole chromosome of annotations. For visualizing smaller genic regions the NetAffx IGB links should be used.

Design Time Annotation Files

HuEx-1_0-st-v2 Annotations, Full, Hg16/Build 34, GFF (324 MB, 1/13/06)

HuEx-1_0-st-v2 Annotations, Full, Hg17/Build 35, GFF (324 MB, 1/13/06)

HuEx-1_0-st-v2 Annotations, Full, Hg18/Build 36, GFF (323 MB, 6/20/06)

HuEx-1_0-st-v2 Annotations, Probeset, Hg16/Build34, CSV (41 MB, 10/6/06)

HuEx-1_0-st-v2 Annotations, Probeset, Hg17/Build35, CSV (41 MB, 10/6/06)

HuEx-1_0-st-v2 Annotations, Probeset, Hg18/Build36, CSV (41 MB, 10/6/06)

HuEx-1_0-st-v2 Mappings, TSV (3.3 MB, 10/11/05)

HuEx-1_0-st-v2 Transcript Cluster ID Mappings to HG-U133 Plus 2.0 (405 KB, 8/28/06)

Note: For gene level analysis you will need to download one of the probeset level annotation zip files (CSV) to get the meta-probeset lists. None of these files contain textual biological annotations such as gene name or GO annotations; textual biological information can be found in the NetAffx Transcript CSV file. The design time Probeset CSV file provides information about the probeset such as genome coordinate and supporting annotation types. This is a subset of information found in the NetAffx Probeset CSV file. The Probeset CSV file is also a subset of the Full GFF files which contain transcript cluster, exon cluster, probeset, and probe level annotations. The GFF files also contain information about the specific annotations supporting each of the probesets. For example, use the Full GFF files for pulling design time information into a local database. The Mappings TSV file contains mappings between other arrays and annotations.

The transcript cluster mapping files provide gene level mappings between 3' IVT probesets and exon array transcript cluster IDs.

Current NetAffx Annotation Files

HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 32 (40 MB, 6/23/11)

HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 32 (26 MB, 6/23/11)

Note: NetAffx annotation files are intended for use in automated analysis. Some are not compatible with all spreadsheet applications. Please use the NetAffx Analysis Center to limit download data to your probe sets of interest. Annotation CSV files for the exon arrays are split into a probe set-level annotation file and a transcript cluster-level annotation file; exon array CSV files are compatible with ExACT.