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Eureka™ Genotyping Solution for Agrigenomics–Targeted genotyping by sequencing

Accurate and affordable targeted genotyping by sequencing (GBS) for agrigenomics.

Agricultural targeted genotyping solutions from Affymetrix offer customized and affordable genotyping tools for highly accurate identification, high throughput and routine screening of genetic markers associated with desired genetic traits and traceability.

Targeted genotyping by sequencing

Eureka™ Genotyping Solution for agrigenomics includes next-generation sequencing (NGS)-based assays designed using Eureka myDesign™ or catalog panels. The Eureka genotyping assay is a fast workflow, going from extracted DNA to genotypes in as few as two days. The complete solution includes genotyping panels, reagent kits, a fully automated workflow, and easy-to-use data analysis tools.

 

Eureka™ Genotyping Solution

Powerful solution.
Your content. Rapid, flexible and accurate answers.

Genotype any species, genome size, or ploidy level with your own proprietary markers or markers from recent sequencing initiatives on the fully customizable Eureka genotyping platform.

Rapid: Two-day workflow goes from extracted DNA to genotypes for thousands of samples per week.

Targeted: The custom design process and assay workflow ensures that all informative loci are targeted across all samples and across all manufacturing batches.

Flexible: Eureka genotyping panels can easily be updated to add or remove variants.

Affordable: The low cost per base of NGS is achieved through an intelligent combination of locus and allelic barcode and sample multiplexing.

Fully customizable Eureka genotyping solution

 

Accurate and affordable targeted genotyping by sequencing for agrigenomics.
     LeavesSeedsBlood spotsEar tagsCrude lysate

Multiple sample types

Supports multiple sample types including crude lysate, dried blood spots, leaves, seeds and ear tags.

 

Overview of the Eureka™ Genotyping Solution workflow.

A Eureka™ genotyping panel contains probes that interrogate each marker of interest.
Day 1: DNA is fragmented. Polymorphic sites in the fragmented genomic DNA are interrogated by locus- and allele-specific probes through hybridization.
Day 2: Adjacent probes are ligated. Sample indices are added to each well to encode each sample followed by PCR amplification and pooling of samples. The libraries are sequenced.

Overview of the Eureka Genotyping Solution workflow

 

For Research Use Only. Not for use in diagnostic procedures.

Fast Turnaround Time Automated genotype calling Ease of use
Eureka Genotyping Solution Automated genotype Calling with Eureka Genotyping Analysis suite Easy to use solution for genotyping by sequencing
  • From genomic DNA to genotypes in two days
  • Higher throughput, more samples in less time due to high level of sample multiplexing
  • Short-cycle sequence data generated
  • No manual genotype assignment necessary
  • Eureka™ Analysis Suite: analysis capabilities for automated allele calling and quality assessment of genotypes.
  • Easy results sharing with the flexible software workflow with simplified data management
  • Validated Eureka™ 1.0 Reagent Kit
  • For multiple sample types, such as crude lysate, hair follicles, leave and seeds.
  • Simple workflow easy to implement in large production environments

 

For Research Use Only. Not for use in diagnostic procedures.

Fast assay workflow

  • Starting with as little as 42 ng/µL of genomic DNA, samples are processed in a 384-microtiter plate to generate NGS libraries.
  • The NGS libraries from each 384-plate are pooled, and short-cycle sequence data is generated from the pooled libraries.
  • The assay requires common lab equipment and very little hands-on time.
  • Workflow is easy to implement in production environments where large numbers of samples need to be genotyped with high accuracy in a timely manner.
Targeted genotyping by sequencing By Eureka Genotyping Assay

 

For Research Use Only. Not for use in diagnostic procedures.