Introducing Anatrace® Neopentyl Glycol (NG) class detergents: the next evolution in crystallization.
This new class of detergents, available exclusively from Affymetrix, is based on a family of amphiphiles first described in a recent Nature Methods paper titled, "Maltose?neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins? by groups from Jean-Luc Popot, Brian Kobilka, and Samuel H. Gellman labs.
When membrane-bound proteins are removed from their natural state and solubilized in aqueous environments, the hydrophobic nature of non-polar regions of the protein cause it to unfold. This phenomenon creates many difficulties for those studying membrane protein structure and function.
Our new Anatrace NG detergent class offers a promising alternative to improve protein structural stability and increase the likelihood of crystallization. We recommend these new products to replace conventional detergents such are LM, DM, or OG.
In the Nature Methods paper, a maltose?neopentyl glycol (MNG), such as the new Anatrace Lauryl Maltose Neopentyl Glycol (NG310), demonstrates significant improvements over conventional detergents such as DDM in stabilizing a diverse set of membrane proteins post-extraction.
The authors also looked at the detergent?s extraction capabilities. Lauryl Maltose Neopentyl Glycol showed an ability to extract individual proteins as well as protein quaternary structures. The NG class detergent stabilized the protein and kept the structure intact longer over time compared to DDM and other conventional detergents.
Another exciting feature of this new class of detergents investigated is the ability to facilitate crystal formation. Again, compared to DDM, Lauryl Maltose Neopentyl Glycol established its superiority in creating the necessary stability for crystal growth in solution and via lipidic cubic phase (LCP).
The NG class detergent advantage is derived from its unique molecular architecture. The detergent is built around a central quaternary carbon atom (Fig. 1). The carbon attaches two maltose units together to form a hydrophilic portion with two n-decyl chains on the other end to create a lipophilic side. The central quaternary carbon is thought to be constrained.
This feature, in combination with the two polar heads and two non-polar tails, creates a less dynamic micelle. It possesses a critical micelle concentration 17-fold less than the equivalent maltoside or glucoside (Fig. 2).
These chemical properties are unique to the NG class detergents and directly contribute to increases in protein structural stability and the promise of crystallization.
Discover the difference these products can make in your research.