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For efficient sample quantification of both high and low expressing targets, USB® VeriQuest® qPCR Master Mixes simply offer greater sensitivity for all your qPCR needs.

With our VeriQuest master mixes,

  • Detect as few as 2 copies of your target
  • High efficiency PCR across the full dynamic range
  • Optimal performance creates sensitive results

VeriQuest Probe qPCR Master Mix sensitivity of limited target detection
Simultaneous detection of both low and high expression targets. Mean Ct values (top) and standard curve (bottom) from real-time PCR for a dilution series of human genomic DNA. The single copy gene, RNaseP, was amplified from 6.6-66 pg human gDNA, which corresponds to ~2-20 copies of target gene.


VeriQuest Real-Time qPCR Master Mixes

USB VeriQuest qPCR Master Mixes - available in SYBR® Green or Probe - offer simply greater performance for all you qPCR needs. Quality results from both standard and fast cycling qPCR are attainable with our optimized master mix formulation. VeriQuest master mixes contain all components necessary for real-time PCR reactions in a pre-measured and often single-tube formulation. Simply add DNA template, primers, and water, and the reactions are ready for cycling.

High sensitivity and precision in limited target quantification with VeriQuest Fast Probe qPCR master mix
Amplification plot (top) and standard curve (bottom) from real-time PCR for a 1.33 to 10-fold dilution series of 10 ng to 1 ng of cDNAs reverse-transcribed from HeLa total RNA.


The proprietary reaction buffer, which includes UDG for carry-over contamination prevention, and VeriQuest Taq Polymerase enhance qPCR amplification by reducing primer-dimer formation and non-template priming. These features increase specificity and sensitivity for consistent performance.

VeriQuest Real-Time qPCR Master Mixes - standard mode

  • Consistency over a broad dynamic template concentration range allowing 7 orders of magnitude linear detection
  • Single-tube, TaqMan® Probe and SYBR Green master mix formulations available for qPCR
  • Exceptional, robust performance even with challenging GC-rich regions
  • Highly stable qPCR master mix can remain at room temperature for 72 hours in a pre-assembled reaction
  • Enhanced amplification sensitivity and accuracy of low copy number detection
  • Carry-over contamination prevention with UDG
  • Exceptional reproducibility across 96-wells
  • One-tube master mix - just add template, primers, and water

Standard Mixes
VeriQuest SYBR Green qPCR Master Mix (2X)

VeriQuest SYBR Green qPCR Master Mix with Fluorescein (2X)

VeriQuest Probe qPCR Master Mix (2X)

VeriQuest Probe qPCR Master Mix, No Reference Dye (2X)

VeriQuest Real-Time qPCR Master Mixes - fast mode

  • Optimized for fast mode thermal cycling conditions for results in one-third of the time
  • Reproducibility and consistency over a broad dynamic range: > 8 orders of magnitude linear detection range
  • Exceptional, robust performance even with challenging GC-rich regions
  • Sensitivity and precision with limited targets
  • Highly stable master mix can remain at room temperature for 72 hours in a pre-assembled reaction
  • Contains dUTP and uracil-DNA glycosylase for carryover contamination prevention
  • Exceptional results with performance comparisons

Fast Mixes
VeriQuest Fast SYBR Green qPCR Master Mix (2X)

VeriQuest Fast SYBR Green qPCR Master Mix with Fluorescein (2X)

VeriQuest Fast Probe qPCR Master Mix (2X)

VeriQuest Fast Probe qPCR Master Mix, No Reference Dye (2X)

VeriQuest Real-Time Reverse Transcription qRT-PCR Master Mixes

USB VeriQuest One-Step qRT-PCR Master Mixes are ready-to-use master mixes for RNA quantification. USB VeriQuest One-Step qRT-PCR Master Mixes provide maximum convenience for a real-time, quantitative analysis of RNA templates in a single reaction format.

  • Consistency over a broad dynamic template concentration range allowing 7 orders of magnitude linear detection
  • Multiple platform compatibility with all real-time master mixes
  • One-step, sequential reaction format for minimum hands on time
  • Easily perform analysis of single or duplex targets from multiple RNA samples
  • Pre-mixed format with VeriQuest qRT-PCR mixes reduces potential contamination
  • Exceptional stability even after 10 freeze-thaw cycles

qRT-PCR Mixes
VeriQuest SYBR Green One-Step qRT-PCR Master Mix (2X)

VeriQuest SYBR Green One-Step qRT-PCR Master Mix with Fluorescein (2X)

VeriQuest Probe One-Step qRT-PCR Master Mix (2X)

VeriQuest Probe One-Step qRT-PCR Master Mix, No Reference Dye (2X)

Have confidence in your real-time PCR results with VeriQuest Master Mixes.