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GeneChip WT Terminal Labeling Kit

The GeneChip® WT (Whole Transcript) Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip® WT Sense Target (ST) Labeling Assay.

Part # Description Unit Size Your Price (USD)
Qty
900670
GeneChip WT Terminal Labeling Kit
sufficient for 10 reactions    Please Inquire
900671
GeneChip WT Terminal Labeling Kit
sufficient for 30 reactions    Please Inquire
900652
GeneChip WT Sense Target Labeling and Control Reagents
sufficient for 30 reactions    Please Inquire
     

GeneChip WT Sense Target Labeling and Control Reagents (900652) and GeneChip WT cDNA Synthesis and Amplification Kits (900672 and 900673) are being discontinued in June 2010.

The Whole Transcript (WT) Sense Target Labeling Assay Has an Updated Protocol.

The GeneChip® WT (Whole Transcript) Terminal Labeling Kit is optimized to be used for the fragmentation and labeling steps of the GeneChip® WT Sense Target (ST) Labeling Assay.

The WT Terminal Labeling Kit employs a strategy for reproducible DNA fragmentation with a combination of uracil DNA glycosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE 1). The typical input material is random-primed, single-stranded DNA, which can be obtained using the GeneChip® WT cDNA Synthesis and Amplification Kit (available separately from Affymetrix) that incorporates deoxyuridine at predefined ratios relative to thymidine.

Following fragmentation, the resulting fragmented DNA can then be labeled using terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound, GeneChip® DNA Labeling Reagent. Both components are also provided in the WT Terminal Labeling Kit. The final labeled DNA target is ready to be added to the cocktail for hybridizing to the arrays such as the GeneChip® Human Exon 1.0 ST Array.

Kit Components

Component name
For 10 Rxns
For 30 Rxns
10X cDNA Fragmentation Buffer 48 µL 144 µL
UDG, 10 U/µL 10 µL 30 µL
APE 1, 1,000 U/µL 10 µL 30 µL
5X TdT Buffer 120 µL 360 µL
TdT, 30 U/µL 20 µL 60 µL
DNA Labeling Reagent, 5 mM 10 µL 30 µL
RNase-free Water 825 µL 2x 825 µL

The GeneChip® WT Terminal Labeling Kit is also available as a component of the GeneChip® WT Sense Target Labeling and Control Reagents Kit.

For research use only. Not for use in diagnostic procedures.

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Application Notes

Whole-transcript Expression Analysis (pdf, 557 kb)

Data Sheets

Exon Array System (pdf, 416 KB)

FAQs

Exon Array Analysis

Exon Array Design

WT Sense Target Labeling Assay

Package Inserts

WT Terminal Labeling Kit (pdf, 259 KB)

Safety Data Sheets

WT Terminal Labeling Kit (zip, 141 KB)

Technical Notes

Array Design for the Human Exon 1.0 ST Array (pdf, 348 KB)

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