GeneChip® miRNA Arrays
The GeneChip® miRNA Arrays are powerful tools for studying the role of small non-coding RNA (miRNA, snoRNA, and scaRNA) and their importance in cancer and other diseases.
Since their discovery in 1993, small non-coding RNAs (microRNA, scaRNA, and snoRNA) have emerged as a major component in the regulatory circuitry that underlies the development and physiology of complex organisms.
As a result, it is becoming increasingly important to complement messenger RNA (mRNA) gene expression studies with miRNA analysis to understand the biological context of differentially expressed genes. These key functional gene products are estimated to regulate approximately 30 percent of all protein-coding genes.
To keep pace with the discovery of new and novel miRNA, Affymetrix is pleased to announce the release of the NEW GeneChip® miRNA 3.0 Array to our growing catalogue of miRNA arrays. This new array offers updated content with the same high performance as the previous generation array.
Unique array content of GeneChip® miRNA Arrays
GeneChip miRNA Arrays provide the most sensitive, accurate, and complete measurement of small non-coding RNA transcripts involved in gene regulation.
These are the only arrays with:
- 100 percent miRBase coverage, all organisms.snoRNAs and scaRNAs
- Probe sets unique to pre-miRNA hairpins (miRNA 2.0 and 3.0).
| Performance specifications | miRNA | miRNA 2.0 | miRNA 3.0 |
|---|---|---|---|
|
Reproducibility (inter and intra lot) |
>0.95 |
>0.95 |
>0.95 |
|
Transcripts detected at 1.0 amol |
85% |
85% |
85% |
|
Dynamic range |
>3 logs |
>3 logs |
>3 logs |
|
Total RNA input |
100-500 ng |
100-500 ng |
130-500 ng |
| Array contents | miRNA | miRNA 2.0 | miRNA 3.0 |
|---|---|---|---|
|
miRBase Version |
11 |
15 |
17 |
|
Organisms |
74 |
131 |
153 |
|
Total Probe Sets |
7,815 |
15,644 |
19,724 |
|
Probes/Probe Set for mature miRNA |
4 |
9 |
9 |
|
Human mature miRNA |
847 |
1,105 |
1,733 |
|
Mouse mature miRNA |
609 |
722 |
1,111 |
|
Rat mature miRNA |
351 |
389 |
680 |
|
Human snoRNA and scaRNA |
922 |
2,334 |
2,216 |
|
Human pre-miRNA |
- |
1,105 |
1,658 |
|
Mouse pre-miRNA |
- |
690 |
855 |
|
Rat pre-miRNA |
- |
389 |
486 |
For research use only. Not for use in diagnostic procedures.
Required Products