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GeneChip Mouse Tiling 2.0R Array Set

The GeneChip® Mouse Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments.

Part # Description Unit Size Your Price (USD)
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900852
GeneChip Mouse Tiling 2.0R Array Set
Complete A-G genome set - 7 Arrays    Please Inquire
     

Most comprehensive whole-genome array set for studying protein/DNA interactions in chromatin immunoprecipitation (ChIP) experiments.

  • High-density 35 base pair probe spacing offers a more accurate view of protein/DNA interactions
  • 25-mer probes yield the most specific hybridization results available

The GeneChip® Mouse Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments. This set of seven arrays contains approximately 45 million oligonucleotide probes to interrogate the mouse genome. Each array in the set contains over 6.5 million perfect match probes to specifically interrogate genomic regions.

Sequences used in the design of the Mouse Tiling 2.0R Array Set were selected from NCBI mouse genome assembly (Build 33). Repetitive elements were removed by RepeatMasker. The Mouse Tiling 2.0R Array Set is available as a whole-genome set of seven arrays (P/N 900852).

The GeneChip® Mouse Promoter 1.0R Array is a subset of the Mouse Tiling 2.0R Tiling Array Set, and the content was chosen specifically to analyze protein/DNA interactions within promoter regions.

Probes are tiled at an average resolution of 35 base pairs, as measured from the central position of adjacent 25-mer oligos, leaving a gap of approximately 10bp between probes. Oligonucleotide probes are synthesized in situ complementary to each corresponding sequence.

Instrument and Software Requirements

Visit the Affymetrix ChIP-on-Chip Web Forum and share protocols, experiences, hints and tips on best practices for ChIP-on-chip and MeDIP-on-chip applications. Free registration is required to view or create posts.

For research use only. Not for use in diagnostic procedures.

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Application Notes

ChIP-on-chip (pdf, 487 KB)

Data Sheets

Human Tiling Arrays (pdf, 260 KB)

Library Files

Mouse Tiling 2.0R Array Set (zip, 1 GB)

GeneChip Tiling Arrays - Data Analysis Library File Updates

Mm35b_P Mouse Genome Tiling 2.0 Array Set NCBIv36 BPMAP files (620 MB 12/14/07)

Manuals

GeneChip® Eukaryotic Double Strand Whole Transcript Protocol - for human and mouse tiling arrays (pdf, 160 KB)

GeneChip® Eukaryotic Double Strand Whole Transcript Protocol - for Arabidopsis, C. elegans, Drosophila, and yeast tiling arrays (pdf, 174 KB)

Affymetrix® Chromatin Immunoprecipitation Assay Protocol (pdf, 424 KB)

Package Inserts

Mouse Tiling 2.0R Array Set (pdf, 247 KB)

Safety Data Sheets

WT Double-Stranded cDNA Synthesis Kit (zip, 166 KB)

Tutorials

Tiling Analysis Software Tutorial

Archived Presentations

Webinar, Six-part ChIP-on-chip Symposia Series

Scientific Forum, 1st European ChIP-on-chip

Other

Fluidics Scripts

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