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GeneChip Human Tiling 2.0R Array Set

The GeneChip Human Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments.

Part # Description Unit Size Your Price (USD)
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900772
GeneChip Human Tiling 2.0R Array Set
Complete A-G genome set 1 7-Array set    Please Inquire
     

Most comprehensive whole-genome array set for studying protein/DNA interactions in chromatin immunoprecipitation (ChIP) experiments.

  • High-density 35 base pair probe spacing offers a more accurate view of protein/DNA interactions
  • 25-mer probes yield the most specific hybridization results available

Array Profile

The GeneChip® Human Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments. This set of seven arrays contains approximately 45 million oligonucleotide probes to interrogate the human genome. Each array in the set contains over 6.5 million perfect match probes to specifically interrogate genomic regions.

Sequences used in the design of the Human Tiling 2.0R Array Set were selected from NCBI human genome assembly (Build 34). Repetitive elements were removed by RepeatMasker. The Human Tiling 2.0R Array Set is available either as a whole-genome set of seven arrays (P/N 900772), or individual arrays from the set may be purchased separately. The GeneChip® Human Promoter 1.0R Array is a subset of the Human Tiling 2.0R Tiling Array Set, and the content was chosen specifically to analyze protein/DNA interactions within promoter regions.

Probes are tiled at an average resolution of 35 base pair, as measured from the central position of adjacent 25-mer oligos, leaving a gap of approximately 10bp between probes. Oligonucleotide probes are synthesized in situ complementary to each corresponding sequence.

Instrument and Software Requirements

GeneChip® Scanner 3000 7G
GeneChip® Operating Software (GCOS) version 1.3 or higher

Visit the Affymetrix ChIP-on-Chip Web Forum (login required) and share protocols, experiences, hints and tips on best practices for ChIP-on-chip and MeDIP-on-chip applications. Free registration is required to view or create posts.

For research use only. Not for use in diagnostic procedures.

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Application Notes

ChIP-on-chip (pdf, 487 KB)

Data Sheets

Human Tiling Arrays (pdf, 260 KB)

Library Files

Human Tiling 2.0R Array Set (zip, 1 GB)

GeneChip Tiling Arrays - Data Analysis Library File Updates

Hs35b_P Human Genome 2.0 Tiling Array Set NCBIv36 BPMAP files (662 MB 12/14/07)

Manuals

GeneChip® Eukaryotic Double Strand Whole Transcript Protocol - for human and mouse tiling arrays (pdf, 160 KB)

GeneChip® Eukaryotic Double Strand Whole Transcript Protocol - for Arabidopsis, C. elegans, Drosophila, and yeast tiling arrays (pdf, 174 KB)

Affymetrix® Chromatin Immunoprecipitation Assay Protocol (pdf, 424 KB)

Package Inserts

Human Tiling 2.0R Array Set (pdf, 489 KB)

Safety Data Sheets

WT Double-Stranded cDNA Synthesis Kit (zip, 166 KB)

Tutorials

Tiling Analysis Software Tutorial

Archived Presentations

Webinar, Six-part ChIP-on-chip Symposia Series

Scientific Forum, 1st European ChIP-on-chip

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