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GeneChip Human Promoter 1.0R Array

Single array to study protein/DNA interactions in over 25,500 human promoters.

Part # Description Unit Size Your Price (USD)
Qty
900775
GeneChip Human Promoter 1.0R Array
contains 2 arrays    Please Inquire
900776
GeneChip Human Promoter 1.0R Array
contains 6 arrays    Please Inquire
900777
GeneChip Human Promoter 1.0R Array
contains 30 arrays    Please Inquire
     

Single array to study protein/DNA interactions in over 25,500 human promoters

  • High-density 35 bp probe spacing offers more probes per promoter for the most accurate view of protein/DNA interactions
  • Extensive 10 kb coverage for each promoter region ensures the most comprehensive promoter analysis
  • 25-mer probes yield the most specific hybridization results available
  • Economical single array design you can use in your own lab

The GeneChip® Human Promoter 1.0R Array is designed for chromatin immunoprecipitation (ChIP) experiments. The Human Promoter Array is a single array comprised of over 4.6 million probes tiled through over 25,500 human promoter regions. Sequences used in the design of the Human Promoter array were selected from NCBI human genome assembly (Build 34). Repetitive elements were removed by RepeatMasker. Promoter regions were selected using sequence information from:

  • 35,685 ENSEMBL genes (version 21_34d, May 14, 2004)
  • 25,172 RefSeq mRNAs (NCBI GenBank®, February 7, 2004)
  • 47,062 complete-CDS mRNA (NCBI GenBank, December 15, 2003)

Probes are tiled at an average resolution of 35 bp, as measured from the central position of adjacent 25-mer oligos, leaving a gap of approximately 10 bp between probes. Each promoter region covers approximately 7.5 kb upstream through 2.45 kb downstream of 5' transcription start sites. For over 1,300 cancer-associated genes, coverage of promoter regions was expanded to include additional genomic content. This more extensive coverage spans from 10 kb upstream through 2.45 kb downstream of transcriptional start sites.

The array interrogates regions proximal to transcription start sites and contains probes for approximately 59 percent of CpG islands annotated by UCSC in NCBI human genome assembly (Build 34). The probes selected for the Human Promoter 1.0R Array are a subset of the probes used in the whole-genome ChIP array set, the GeneChip® Human Tiling 2.0R Array Set (P/N 900772).

For research use only. Not for use in diagnostic procedures.

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Application Notes

ChIP-on-chip (pdf, 487 KB)

Data Sheets

Human Tiling Arrays (pdf, 260 KB)

Library Files

Human Promoter 1.0R Array (zip, 132 MB)

Human Promoter 1.0R Array (zip, 132 MB)

GeneChip Tiling Arrays - Data Analysis Library File Updates

Hs_PromP Human Promoter Array 1.0 R NCBIv36 Annotation Bundle (2 MB 02/07/08)

Human Promoter NCBIv36 Annotation Bundle

Human Promotor Tiling Array NCBIv36 BPMAP file

Hs_PromP Human Promotor Tiling Array NCBIv36 BPMAP file (67 MB 12/14/07)

Manuals

GeneChip® Eukaryotic Double Strand Whole Transcript Protocol - for human and mouse tiling arrays (pdf, 160 KB)

GeneChip® Eukaryotic Double Strand Whole Transcript Protocol - for Arabidopsis, C. elegans, Drosophila, and yeast tiling arrays (pdf, 174 KB)

Affymetrix® Chromatin Immunoprecipitation Assay Protocol (pdf, 424 KB)

Package Inserts

Human Promoter 1.0R Array (pdf, 489 KB)

Tutorials

Tiling Analysis Software Tutorial

Archived Presentations

Webinar, Six-part ChIP-on-chip Symposia Series

Scientific Forum, 1st European ChIP-on-chip

Other

Fluidics Scripts

CEL Files, Human Promoter 1.0R Array (zip, 84 MB)

NetAffx Annotation Files

Human Promoter NCBIv36 Annotation Bundle

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