GeneChip® Human Exon ST Array
With approximately four probes per exon and roughly 40 probes per gene, the GeneChip® Human Exon 1.0 ST Array enables two complementary levels of analysis - gene expression and alternative splicing.
With approximately four probes per exon and roughly 40 probes per gene, the GeneChip® Human Exon 1.0 ST Array enables two complementary levels of analysis—gene expression and alternative splicing.
Multiple probes per exon enable "exon-level" analysis and allow you to distinguish between different isoforms of a gene. This exon-level analysis on a whole-genome scale opens the door to detecting specific alterations in exon usage that may play a central role in disease mechanism and etiology.
The second level is "gene-level" expression analysis, in which multiple probes on different exons are summarized into an expression value of all transcripts from the same gene.
Exon arrays provide the most comprehensive coverage of the genome, including empirically supported and predicted transcribed sequences, enabling the discovery of previously unidentified novel events.
Features of this integrated solution include:
- Highest resolution expression analysis, interrogating over 1 million exon clusters within the known and predicted transcribed regions of the entire genome. Read more >
- Tailored GeneChip® Whole Transcript (WT) Assay and Reagents utilizing a random priming method for generating sense targets along the entire length of the transcripts.
- Flexible data analysis solutions and comprehensive annotation, giving you the opportunity to explore and dissect the data in various user-defined workflows.
Explore the Exon Array Design
GeneChip® Exon Array Sample Data
Technical Note, Array Design for the Human Exon 1.0 ST Array (pdf, 348 KB)
Technical Note, Identifying and Validating Alternative Splicing Events
New XRAY demos for exon expression analysis
|Design Statistics Summary|
|Probe Sets||1.4 million|
|Exon Clusters||> 1 million|
|Supported by Putative Full-Length mRNA||289,961 probe sets|
|Supported by Ensembl Transcripts||306,583 probe sets|
|Supported by EST||665,175 probe sets|
|Supported by Mouse or Rat mRNA||220,262 probe sets|
|Supported by Gene Prediction||883,105 probe sets|
|Probe Selection Region||Along the entire length of the transcripts|
|Probes/Probe Selection Region||4¹|
|Background Subtraction Strategy||Median intensity of up to 1,000 background probes with the same GC content|
|Total Features Per Array||>5,500,000|
¹ About 10 percent of the exon probe sets have fewer than four probes due to the probe selection region length and sequence constraints.
²The probes tiled on the array are designed in the anti-sense orientation, requiring sense-strand labeled targets to be hybridized to the array. By convention, the array is called ST array representing the necessity of using sense targets (the labeled samples to be hybridized to the array).
For research use only. Not for use in diagnostic procedures.
|GeneChip® Human Gene ST Arrays|
|Affymetrix® Human Gene ST Array Plates|