PrepEase Yeast Plasmid Isolation Kit
The PrepEase®Yeast Plasmid Isolation Kit is designed for the isolation of 2μ plasmids from either yeast patches on plates or from yeast grown in small liquid culture. The isolation protocol eliminates the need for phenol/chloroform extraction, glass bead disruption and column elution.
The isolated plasmid DNA can be used for PCR amplification, bacterial transformation or for direct sequencing. The ability to sequence directly from the isolated plasmid without the need for bacterial transformation or a pre-amplification step and restriction enzyme digestion makes it an efficient method for screening yeast transformants. The kit may also be used to obtain spheroplasts for other analysis.
|Sequencing results from yeast plasmid isolated from A) single yeast patch on a plate and B) 1.5 ml of yeast culture.|
Minimize the time required to analyze yeast plasmids, e.g. yeast two-hybrid experiments. The need to shuttle plasmids from yeast to E. coli is eliminated.
Allows the isolation of plasmid from yeast cultures almost as rapidly and readily as from E. coli.
No organic extraction or mechanical disruption of yeast samples is involved.
Yields plasmid DNA suitable for a variety of applications, including direct sequencing, PCR amplification, and transformation of chemically competent bacterial cells.
Shipped on dry ice. Store at -20°C.