Ligate-IT Rapid Ligation Kit
Can be used for a variety of ligation procedures such as conventional vector cloning, TA cloning, linker or adaptor ligation, and library construction
Ligate-IT? Rapid Ligation Kit provides high-quality reagents for fast and easy ligation reactions.
Quick and Efficient
Ligation reactions are complete following room temperature incubation in just 5 minutes for cohesive-ends and 10 minutes for blunt-ends (see Figs. 1-2). Transform competent cells immediately or store reactions at -20?C for later transformation.
Ligate-IT Rapid Ligation Kit includes USB T4 DNA Ligase, Nuclease-Free Water, and a specially formulated 5X Reaction Buffer which enhances the activity of T4 DNA Ligase, especially on blunt-end fragments. All of the components are in a ready-to-use format and do not require any additional reagents, such as ATP.
The kit can be used for a variety of ligation procedures such as conventional vector cloning, TA cloning, linker or adaptor ligation, and library construction.
Reaction 100 Reaction
Pack Size Pack Size
Ligate-IT T4 DNA Ligase (PN 78401) 25 ?l 100 ?l
5X Ligate-IT Reaction Buffer (PN 78402) 100 ?l 400 ?l
Water, Nuclease-Free (PN 71786) 1 ml 2 x 1 ml
Recombinant T4 DNA Ligase is supplied in 25mM Tris-HCl (pH 7.6), 100mM NaCl, 1mM DTT, 0.5mM EDTA, 50% glycerol.
Kit shipped on dry ice. Store components at -20?C in a non-frost-free freezer.
Tested User Friendly? Functional Test:
Cohesive- and blunt-end ligations of a 1200 bp insert into pUC19 yielded ?1 x 106 transformants per microgram of linearized, dephosphorylated vector.
1. SAMBROOK, J. AND RUSSELL, D. W. (2001) ?Molecular Cloning: A Laboratory Manual,? Cold Spring Harbor Laboratory Press, 1.84-1.125.