RapidGel, 40% Liquid Acrylamide Stock Solution
Pre-mixed, 40% liquid acrylamide (19:1 acrylamide:bis-acrylamide ratio) in ready-to-use form for Polyacrylamide Gel Electrophoresis (PAGE). Stock solution provides flexibility for different acrylamide percentages in a wide variety of gel applications. This formulation does not contain urea.
Functionally tested in gel electrophoresis.
Dilute 40% stock solution to desired concentration with appropriate running buffer for PAGE. To make 100 ml of a sequencing gel solution containing 7-8.3M urea, add an appropriate volume of 40% RapidGel solution, 10 ml of 10X (or 5 ml of 20X) running buffer, 42-50 gm of urea and high purity dH2O to 100 ml in a clean, graduated cylinder. Stir and warm solution at 35-45°C to dissolve urea. (Do not microwave.) Cool to room temperature. Filter through a 0.2 μm filter and leave under gentle vacuum for 10 min to de-gas. Add 600 μl 10% ammonium persulfate and 60 μl TEMED per 100 ml gel solution to polymerize. Allow to polymerize at room temperature from one hour to overnight. For polymerization within 30-45 min, add 1 ml 10% ammonium persulfate and 100 μl TEMED per 100 ml gel solution. Pour gel immediately.
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