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USB® Taq DNA Polymerase

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Economical thermostable enzyme with no contamination and 5' → 3' exonuclease activity, suitable for routine PCR

Part # Description Unit Size
74165 50 UN
USB Taq DNA Polymerase
50 units   
74165 400 UN
USB Taq DNA Polymerase
400 units   
74165 2000 UN
USB Taq DNA Polymerase
2,000 units   
74165 4000 UN
USB Taq DNA Polymerase
4,000 units (2 x 2,000 units)   
74165 20000 UN
USB Taq DNA Polymerase
20,000 units   

Source:

Recombinant

Description:
USB® Taq DNA Polymerase is a thermostable enzyme designed for routine PCR and suitable for real time qPCR. USB Taq is vigorously tested and has no detectable contaminating endonucleases or exonucleases, which offers consistent performance and ensures quality results. It has a highly processive 5' → 3' polymerase activity and a 5' → 3' exonuclease activity. USB Taq DNA Polymerase is available in economical pack sizes to meet a range of demands, and is supplied with 10X PCR Reaction Buffer.


USB Taq DNA Polymerase is available for custom formulations or bulk quantities to meet individual specifications or concentrations. This enzyme is sold without third party licensing restrictions on subsequent use, offering the ability to place into kits or master mixes for resale purposes if authorized in writing by Affymetrix.

Lambda DNA with USB Taq DNA Polymerase

Fig 1. DNA fragments ranging from 0.1 kb to 6 kb amplified from λ DNA.


Human gDNA with USB Taq DNA Polymerase

Fig 2. NUMB fragments ranging from 0.2 kb to 4 kb amplified from human genomic DNA.


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Properties:
Activator: Mg2+

Purity:
Free from detectable non-specific nucleases.

Storage Buffer:
20 mM Tris-HCl pH 8.5, 0.1 mM EDTA, 100mM KCl, 1.0 mM DTT, 50% Glycerol, and stabilizers.

Assay Conditions:
The reaction mixture contains 25 mM TAPS, pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-ME, 200 µM each dATP, dGTP, dTTP, 100 µM [α-33P]-dCTP (0.05 to 0.1 Ci/mmole), 250 µg/ml activated salmon sperm DNA and Taq DNA Polymerase. After incubation at 74°C for 10 minutes, acid insoluble material is determined (50 µl reaction volume).

Unit Definition:
One unit of enzyme is defined as the amount that catalyzes the incorporation of 10 nmol of total nucleotide into acid insoluble form in 30 minutes at 74°C.

Concentration:
5 units/µl

Functional Test:
Functionally tested in PCR in amplification of Human gDNA, generating 2016, 972, 447, 268, and 169 bp fragments.

Functionally Tested 10X PCR Reaction Buffer (included, PN 71165):
100mM Tris-HCl (pH 8.6), 500mM KCl, 15mM MgCl2

Applications:

• Routine PCR amplification
• Suitable for real time qPCR

Storage:

Shipped on dry ice. Store at -20°C.

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Brochures

PCR Tools Brochure

MSDS / Material Safety Data Sheets

USB Taq DNA Polymerase SDS

Product Spotlight

USB Taq DNA Polymerase Product Spotlight

Protocol, Brief

USB Taq DNA Polymerase Brief Protocol

Tech Tips

PCR product selection guide

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