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Dextran Sulfate, Sodium Salt

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Part # Description Unit Size
14489 10 GM
Dextran Sulfate, Sodium Salt
10 gm   
14489 50 GM
Dextran Sulfate, Sodium Salt
50 gm   
14489 100 GM
Dextran Sulfate, Sodium Salt
100 gm   
14489 1 KG
Dextran Sulfate, Sodium Salt
1 kg   

Molecular Weight:

40,000 - 50,000

Dextran Sulfate Sodium Salt (DSS) is a polyanionic derivative of Dextran. This DSS induces experimental colitis and promotes colitis-associated cancer in rodents[1]. Inflammatory bowel diseases (IBD), primarily including ulcerative colitis and Crohn's disease, are studied using a variety of chemically induced colitis models in rodents that have become essential to our advancing understanding of these diseases[2]. Using DSS in colitis models, the symptoms of human ulcerative colitis can be replicated fairly accurately and studied systematically.

The trial cases of IBD for the purpose of colitis cancer research commonly rely on the addition of DSS to the rodents drinking water to cause intestinal inflammation. This oral administration of a range of concentrations of DSS produces acute colitis in mice[3] and has been exploited as a model of colonic epithelial injury with many features found in human inflammatory bowel disease (IBD)[4].

In order to properly ensure uniformity of the acute colitis in the study, it is recommended that DSS is purchased in bulk quantities. USB Affymetrix offers 1 KG pack sizes with the option to reserve custom lots in order to decrease the possibility of variations through different vendors and manufacturing lots.

The following journal articles describe the use of USB Dextran Sulfate, Sodium Salt to induce experimental colitis in mice:
Dextran Sodium Sulfate Inhibition of Real-Time PCR Amplification: A Poly-A Purification Solution by Kerr et al[1].
Fucoidan Extracts Ameliorate Acute Colitis by Lean et al[2].

Additional Applications of DSS:

Rapid Hybridization by DSS

  • Dextran sulfate in 10%, originally found to accelerate DNA hybridization in solution by about 10 fold[5], Dextran also accelerate the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100 fold, without increasing the background significantly[6,7].

Immunological studies
  • Phenotype studies of M1 and M2 macrophages by introducing DSS in animal model[8].

PRODUCT SPECIFICATIONS
Form: Off white to light yellow powder
Loss on Drying: ≤ 10.0%
pH (1%, H2O): 5.0 - 7.5
Specific Viscosity (25°C in 1.0 M NaCl): 0.05 - 0.13
Sulfur: 17.0 - 20.0%
Glucose: 35.0 - 48.0%
Residue on Ignition: 35.0 - 50.0%

CAS:

9011-18-1

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References:
1. KERR, T. A., CIORBA, M. A., MATSUMOTO, H., DAVIS, V. R. T., LUO, J., KENNEDY, S., XIE, Y., SHAKER, A., DIECKGRAEFE, B. K. AND DAVIDSON, N. O. (2011) Inflamm Bowel Dis. 18(2), 344-348.

2. LEAN, Q. I., ERI, R. D., FITTON, J. H., PATEL, R. P. AND GUEVEN, N. (2015) PLoS One 10(6): e0128453. doi: 10.1371/journal.pone.0128453.

3. ROBLES, H. V., MADRID, A. F. C., PONCE, A. G., OLIVARES, A. S., SHIBAYAMA, M., BETANZOS, A., DEL VALLE MONDRAGᅮN, L., NAVA, P. AND SCHNOOR, M. (2016) Oxidative Medicine and Cellular Longevity 2016, Article ID 8473242.

4. COOPER, H. S., MURTHY, S. N., SHAH, R. S., AND SEDERGRAN, D. J. (1993) Lab Invest. 69, 238249.

5. WETMUR, J.G. (1975) Biopolymers 14, 2517-2524.

6. WAHL, G. M., et al. (1979) Proc. Natl. Acad. Sci. USA 76, 3683.

7. MANIATIS, T., FRITSCH, E. AND SAMBROOK, F. (1982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory.

8. ZHU, Y., LI, X., CHEN, J., CHEN, T., SHI, Z., LEI, M., ZHANG, Y., BAI, P., LI, Y., AND FEI, X. (2016) International Immunopharmacology 30, 74-84.

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MSDS / Material Safety Data Sheets

Dextran Sulfate, Sodium Salt SDS

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