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Ambion WT Expression Kit

The Ambion® WT Expression Kit, combined with the GeneChip® WT Terminal Labeling and Controls Kit, is the newest, most effective option for target preparation on GeneChip® Exon and Gene 1.0 ST Arrays.

Part # Description Unit Size Your Price(USD)
Qty
901525
GeneChip WT Terminal Labeling and Controls Kit
 sufficient for 10 reactions    Please Inquire
901524
GeneChip WT Terminal Labeling and Controls Kit
 sufficient for 30 reactions    Please Inquire
4411973
Ambion WT Expression Kit
 10 reactions. Order from Ambion    Please Inquire
4411974
Ambion WT Expression Kit
 30 reactions. Order from Ambion    Please Inquire
     

The Ambion® WT Expression Kit, combined with the GeneChip® WT Terminal Labeling and Controls Kit, is the newest, most effective option for target preparation on GeneChip® Exon and Gene 1.0 ST Arrays. The basic chemistry is the same as the Affymetrix® GeneChip® WT Sense Target Labeling and Control Reagents kit; however, the Ambion WT Expression Kit utilizes a streamlined protocol with master mixes, does not require an upfront rRNA-reduction step for optimal performance, and uses optimized reagents that enable lower total RNA input amounts.

The WT Expression Kit is co-marketed by Affymetrix and Ambion and can only be purchased directly from Ambion. The GeneChip® WT Terminal Labeling and Controls Kit, which contains the GeneChip® WT Terminal Labeling Kit, GeneChip® Poly-A Control Kit, and GeneChip® Hybridization Control Kit can be purchased directly from Affymetrix.

Key Features

Key features of the Ambion WT Expression Kit:

  • 50 ng total RNA input
  • No ribosomal removal required for Human, Mouse, and Rat Exon 1.0 ST and Gene 1.0 ST Arrays
  • Streamlined workflow for a simplified protocol and reduced hands-on time
  • Magnetic bead-based purification for enhanced recovery

The Ambion WT Expression Kit includes:

  • All reagents needed to generate sense cDNA ready for fragmentation and labeling
  • Magnetic beads for enhanced purification efficiency
  • Plastic consumables required to run the assay

The Affymetrix GeneChip® WT Terminal Labeling and Controls Kit includes:

  • WT Fragmentation and Labeling Kit required for target produced by the Ambion WT Expression Kit
  • Poly-A controls that are added to input RNA to measure efficiency of target amplification by the Ambion WT Expression Kit
  • Hybridization controls that monitor the hybridization process for troubleshooting purposes

What you need to order

From Ambion

Name Part Number Size
Ambion® WT Expression Kit 4411973 10 reactions
Ambion® WT Expression Kit 4411974 30 reactions

From Affymetrix

Name Part Number Size
GeneChip® WT Terminal Labeling and Controls Kit

Contains:

  • 900670 GeneChip® WT Terminal Labeling Kit (10 reaction)
  • 900433 GeneChip® Poly-A Control Kit
  • 900454 GeneChip® Hybridization Control Kit
 901525 10 reactions*
GeneChip® WT Terminal Labeling and Controls Kit

Contains:

  • 900671 GeneChip® WT Terminal Labeling Kit (30 reaction)
  • 900433 GeneChip® Poly-A Control Kit
  • 900454 GeneChip® Hybridization Control Kit
 901524 30 reactions
GeneChip® Hybridization, Wash, and Stain Kit 900720 30 reactions

*Additional GeneChip® WT Terminal Labeling Kit, 10 reactions (P/N 900670), is available separately.

Documentation

The following documentation is required for using the Ambion WT Expression Kit and the GeneChip® WT Terminal Labeling and Controls Kit with Exon and Gene 1.0 ST Arrays.

Name Part Number
Protocol, Ambion® WT Expression Kit 4425209
User Manual, GeneChip® WT WT Terminal Labeling and Hybridization 702808
User Manual, GeneChip® Expression Wash, Stain and Scan 702731

For research use only. Not for use in diagnostic procedures.

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