GeneChip Human Genome U133A 2.0 Array
The GeneChip® Human Genome U133A 2.0 Array is a single array representing 14,500 well-characterized human genes that can be used to explore human biology and disease processes.
The GeneChip® Human Genome U133A 2.0 Array is a single array representing 14,500 well-characterized human genes that can be used to explore human biology and disease processes. New design and reduced feature size mean that you can use smaller sample volumes than the previous HG®U133A Array without compromising performance.
- Provides coverage of well-substantiated genes in the transcribed human genome on a single array
- Analyzes the expression level of 18,400 transcripts and variants, including 14,500 well-characterized human genes
- Comprised of more than 22,000 probe sets and 500,000 distinct oligonucleotide features
- Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform
- All probe sets represented on the GeneChip® Human Genome U133A Array are identically replicated on the GeneChip Human Genome U133A 2.0 Array
Array Profile
Sequences used in the design of the array were selected from GenBank®, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then were refined by analysis and comparison with a number of other publicly available databases including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).
Instrument and Software Requirements
- GeneChip® Scanner 3000, enabled for High-Resolution Scanning*
- GeneChip® Command Console® Software (AGCC) including the GeneChip® Scanner 3000 High-Resolution Scanning Patch
*GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, serial number series 501, will require the 00-0110 GeneChip Scanner 3000 High-Resolution Update to be installed.
For more information, please review the data sheet (pdf, 169 KB).
For research use only. Not for use in diagnostic procedures.

Application Notes
Microarray Applications in Infectious Disease (pdf, 252 KB)
Microarrays in Cancer Research (pdf, 423 KB)
Data Sheets
Human Genome U133 Arrays (pdf, 1.76 MB)
Library Files
Human Genome U133A 2.0 Array (zip, 13 MB)
Manuals
HT 3' IVT Express Kit (pdf 5.93 MB)
Probe Set Data in MAGE-ML Format
3' IVT Express Kit User Manual (pdf, 1.6 MB)
Mask Files
Human Genome U133A 2.0 Array Normalization Controls (zip, 1 KB)
Package Inserts
Human Genome U133A 2.0 Array (pdf, 492 KB)
Protocol, Long
Gene Expression Assay and Data Analysis
Technical Notes
GeneChip Expression Platform: Comparison, Evolution, and Performance (pdf, 269 KB)
Other
NetAffx Alignment Files
HG-U133A_2 Alignments to Genome, PSL (4 MB 09/28/06)
NetAffx Annotation Files
HG-U133A_2 Annotations, CSV format, Release 30 (13 MB 11/15/09)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 30 (49 MB 11/15/09)
HG-U133A_2 BLASTP annotations, CSV Format, Release 30 (14 KB 11/16/09)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 30 (5 MB 11/15/09)
Sequence Files
HG-U133A_2 Consensus Sequences, FASTA (7 MB 08/20/08)
HG-U133A_2 Control Sequences, FASTA (23 KB 09/29/03)
HG-U133A_2 Exemplar Sequences, FASTA (11 MB 09/29/03)
HG-U133A_2 Probe Sequences, FASTA (5 MB 08/20/08)
HG-U133A_2 Probe Sequences, Tabular (4 MB 08/20/08)
HG-U133A_2 Target Sequences, FASTA (5 MB 08/20/08)
Archived NetAffx Annotation Files
HG-U133A_2 Annotations, MAGE-ML XML, Release 20 (46 MB 06/15/07)
HG-U133A_2 Annotations, CSV, Release 20 (10 MB 05/31/07)
HG-U133A_2 Annotations, BLASTX, Release 20 (2 MB 06/23/04)
HG-U133A_2 Annotations, BLASTP, Release 20 (3 MB 07/14/06)
HG-U133A_2 Annotations, Orthologs, Release 20 (4 MB 08/21/06)
HG-U133A_2 Annotations, CSV format, Release 21 (10 MB 05/31/07)
HG-U133A_2 Annotations, MAGE-ML XML format, Release 21 (47 MB 06/15/07)
HG-U133A_2 BLASTP annotations, CSV format, Release 21 (3 MB 11/15/06)
HG-U133A_2 Orthologs/Homologs, CSV format, Release 21 (4 MB 11/30/06)
HG-U133A_2 Annotations, CSV format, Release 22 (10 MB 05/31/07)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 22 (43 MB 06/15/07)
HG-U133A_2 BLASTP annotations, CSV Format, Release 22 (46 KB 03/09/07)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 22 (5 MB 03/09/07)
HG-U133A_2 Annotations, CSV format, Release 23 (10 MB 07/12/07)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 23 (42 MB 07/12/07)
HG-U133A_2 BLASTP annotations, CSV Format, Release 23 (42 KB 07/12/07)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 23 (4 MB 07/12/07)
HG-U133A_2 Annotations, CSV format, Release 24 (10 MB 11/06/07)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 24 (42 MB 11/06/07)
HG-U133A_2 BLASTP annotations, CSV Format, Release 24 (2 KB 11/06/07)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 24 (4 MB 11/06/07)
HG-U133A_2 Annotations, CSV format, Release 25 (10 MB 03/18/08)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 25 (43 MB 03/18/08)
HG-U133A_2 BLASTP annotations, CSV Format, Release 25 (21 KB 03/18/08)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 25 (4 MB 03/18/08)
HG-U133A_2 Annotations, CSV format, Release 26 (11 MB 07/08/08)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 26 (45 MB 07/08/08)
HG-U133A_2 BLASTP annotations, CSV Format, Release 26 (6 KB 07/08/08)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 26 (5 MB 07/08/08)
HG-U133A_2 Annotations, CSV format, Release 27 (11 MB 11/30/08)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 27 (45 MB 11/24/08)
HG-U133A_2 BLASTP annotations, CSV Format, Release 27 (27 KB 11/24/08)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 27 (5 MB 11/24/08)
HG-U133A_2 Annotations, CSV format, Release 28 (12 MB 03/12/09)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 28 (48 MB 03/12/09)
HG-U133A_2 BLASTP annotations, CSV Format, Release 28 (3 KB 03/04/09)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 28 (5 MB 03/12/09)
HG-U133A_2 Annotations, CSV format, Release 29 (12 MB 07/01/09)
HG-U133A_2 Annotations, MAGE-ML XML Format, Release 29 (48 MB 07/01/09)
HG-U133A_2 BLASTP annotations, CSV Format, Release 29 (3 KB 07/01/09)
HG-U133A_2 Orthologs/Homologs, CSV Format, Release 29 (5 MB 07/01/09)

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