Human Genome U133 Set
The Human Genome U133 Set is available through the Affymetrix Made-to-Order Program.
Note: This is a previous generation product and it can only be ordered in whole lots. This GeneChip array is available through the Affymetrix Made-to-Order Program, which enables you to purchase selected custom array designs and previous-generation GeneChip arrays no longer available as catalog products. Learn more about ordering and support of GeneChip Made-to-Order arrays. Please contact Affymetrix, Inc. at 1-888-DNA-CHIP for lot sizes and additional pricing information.
The Human Genome U133 (HG-U133) Set, consisting of two GeneChip® arrays, contains almost 45,000 probe sets representing more than 39,000 transcripts derived from approximately 33,000 well-substantiated human genes. This set design uses sequences selected from GenBank®, dbEST, and RefSeq.
The sequence clusters were created from the UniGene database (Build 133, April 20, 2001). They were then refined by analysis and comparison with a number of other publicly available databases including the Washington University EST trace repository and the University of California, Santa Cruz Golden Path human genome database (April 2001 release).
The HG-U133A Array includes representation of the RefSeq database sequences and probe sets related to sequences previously represented on the Human Genome U95Av2 Array. The HG-U133B Array contains primarily probe sets representing EST clusters.
For more information, please review the data sheet (pdf, 169 KB).
For research use only. Not for use in diagnostic procedures.

Application Notes
Microarray Applications in Infectious Disease (pdf, 252 KB)
Comparison Sheets / Array Comparisons
HG-U95 to HG-U133, Best Match (zip, 842 KB)
HG-U133 to HG-U133 Plus, Complex (zip, 24 MB)
HG-U133 to HG-U133 Plus, Good Match (zip, 5 KB)
HG-U95 to HG-U133, No Match (zip, 54 KB)
HG-U133 to HG-U133 Plus, Best Match (zip, 5 KB)
HG-U95 to HG-U133, Good Match (zip, 900 KB)
HG-U95 to HG-U133, Complex (zip, 23.3 MB)
HG-U133 to HG-U133 Plus, No Match (zip, 1 KB)
Data Sheets
Human Genome Arrays (pdf, 117 KB)
Standardized Assays and Reagents for GeneChip Expression Analysis (pdf, 3.02 MB)
FAQs
Library Files
Human Genome U133 Set (zip, 25 MB)
Manuals
Probe Set Data in MAGE-ML Format
HT 3' IVT Express Kit (pdf 5.93 MB)
3' IVT Express Kit User Manual (pdf, 1.6 MB)
Mask Files
Human Genome U133A Set Normalization Controls (zip, 1 KB)
Human Genome U133B Set Normalization Controls (zip, 1 KB)
Package Inserts
Human Genome U133 Set (pdf, 532 KB)
Technical Notes
Array Design for the Human Genome U133 Set (pdf, 18 MB)
Manufacturing Quality Control and Validation Studies of GeneChip Arrays (pdf, 3.28 MB)
Performance and Validation of the Human Genome U133 Set (pdf, 42 MB)
Other
Latin Square Data for Expression Algorithm Assessment
NetAffx Alignment Files
HG-U133A Alignments to Genome, PSL (4 MB 09/28/06)
HG-U133B Alignments to Genome, PSL (3 MB 09/28/06)
NetAffx Annotation Files
HG-U133A Annotations, CSV format, Release 30 (12 MB 11/15/09)
HG-U133A Annotations, MAGE-ML XML Format, Release 30 (49 MB 11/15/09)
HG-U133A BLASTP annotations, CSV Format, Release 30 (14 KB 11/16/09)
HG-U133A Orthologs/Homologs, CSV Format, Release 30 (5 MB 11/15/09)
HG-U133B Annotations, CSV format, Release 30 (9 MB 11/15/09)
HG-U133B Annotations, MAGE-ML XML Format, Release 30 (33 MB 11/15/09)
HG-U133B BLASTP annotations, CSV Format, Release 30 (27 KB 11/16/09)
HG-U133B Orthologs/Homologs, CSV Format, Release 30 (3 MB 11/15/09)
Sequence Files
HG-U133A Consensus Sequences, FASTA (7 MB 08/20/08)
HG-U133A Control Sequences, FASTA (23 KB 03/28/03)
HG-U133A Exemplar Sequences, FASTA (11 MB 03/28/03)
HG-U133A Probe Sequences, FASTA (5 MB 08/20/08)
HG-U133A Probe Sequences, Tabular (4 MB 08/20/08)
HG-U133A Target Sequences, FASTA (5 MB 08/20/08)
HG-U133B Consensus Sequences, FASTA (10 MB 08/20/08)
HG-U133B Control Sequences, FASTA (24 KB 03/28/03)
HG-U133B Exemplar Sequences, FASTA (1 MB 03/28/03)
HG-U133B Probe Sequences, FASTA (5 MB 08/20/08)
HG-U133B Probe Sequences, Tabular (4 MB 08/20/08)
HG-U133B Target Sequences, FASTA (4 MB 08/20/08)
Archived NetAffx Annotation Files
HG-U133A Annotations, MAGE-ML XML, Release 20 (46 MB 06/15/07)
HG-U133B Annotations, MAGE-ML XML, Release 20 (34 MB 06/15/07)
HG-U133A Annotations, CSV, Release 20 (10 MB 05/31/07)
HG-U133A Annotations, BLASTX, Release 20 (2 MB 06/23/04)
HG-U133A Annotations, BLASTP, Release 20 (3 MB 07/14/06)
HG-U133A Annotations, Orthologs, Release 20 (4 MB 08/21/06)
HG-U133B Annotations, CSV, Release 20 (8 MB 05/31/07)
HG-U133B Annotations, BLASTX, Release 20 (398 KB 06/23/04)
HG-U133B Annotations, BLASTP, Release 20 (1 MB 07/14/06)
HG-U133B Annotations, Orthologs, Release 20 (3 MB 08/21/06)
HG-U133A Annotations, CSV format, Release 21 (10 MB 05/31/07)
HG-U133A Annotations, MAGE-ML XML format, Release 21 (47 MB 06/15/07)
HG-U133A BLASTP annotations, CSV format, Release 21 (3 MB 11/15/06)
HG-U133A Orthologs/Homologs, CSV format, Release 21 (4 MB 11/30/06)
HG-U133B Annotations, CSV format, Release 21 (8 MB 05/31/07)
HG-U133B Annotations, MAGE-ML XML format, Release 21 (35 MB 06/15/07)
HG-U133B BLASTP annotations, CSV format, Release 21 (1 MB 11/15/06)
HG-U133B Orthologs/Homologs, CSV format, Release 21 (3 MB 11/30/06)
HG-U133A Annotations, CSV format, Release 22 (10 MB 05/31/07)
HG-U133A Annotations, MAGE-ML XML Format, Release 22 (43 MB 06/15/07)
HG-U133A BLASTP annotations, CSV Format, Release 22 (45 KB 03/09/07)
HG-U133A Orthologs/Homologs, CSV Format, Release 22 (4 MB 03/09/07)
HG-U133B Annotations, CSV format, Release 22 (8 MB 05/31/07)
HG-U133B Annotations, MAGE-ML XML Format, Release 22 (33 MB 06/15/07)
HG-U133B BLASTP annotations, CSV Format, Release 22 (90 KB 03/09/07)
HG-U133B Orthologs/Homologs, CSV Format, Release 22 (3 MB 03/09/07)
HG-U133A Annotations, CSV format, Release 23 (10 MB 07/12/07)
HG-U133A Annotations, MAGE-ML XML Format, Release 23 (42 MB 07/12/07)
HG-U133A BLASTP annotations, CSV Format, Release 23 (42 KB 07/12/07)
HG-U133A Orthologs/Homologs, CSV Format, Release 23 (4 MB 07/12/07)
HG-U133B Annotations, CSV format, Release 23 (7 MB 07/12/07)
HG-U133B Annotations, MAGE-ML XML Format, Release 23 (30 MB 07/12/07)
HG-U133B BLASTP annotations, CSV Format, Release 23 (92 KB 07/12/07)
HG-U133B Orthologs/Homologs, CSV Format, Release 23 (2 MB 07/12/07)
HG-U133A Annotations, CSV format, Release 24 (10 MB 11/06/07)
HG-U133A Annotations, MAGE-ML XML Format, Release 24 (42 MB 11/06/07)
HG-U133A BLASTP annotations, CSV Format, Release 24 (2 KB 11/06/07)
HG-U133A Orthologs/Homologs, CSV Format, Release 24 (4 MB 11/06/07)
HG-U133B Annotations, CSV format, Release 24 (8 MB 11/06/07)
HG-U133B Annotations, MAGE-ML XML Format, Release 24 (30 MB 11/06/07)
HG-U133B BLASTP annotations, CSV Format, Release 24 (5 KB 11/06/07)
HG-U133B Orthologs/Homologs, CSV Format, Release 24 (2 MB 11/06/07)
HG-U133A Annotations, CSV format, Release 25 (10 MB 03/18/08)
HG-U133A Annotations, MAGE-ML XML Format, Release 25 (43 MB 03/18/08)
HG-U133A BLASTP annotations, CSV Format, Release 25 (21 KB 03/18/08)
HG-U133A Orthologs/Homologs, CSV Format, Release 25 (4 MB 03/18/08)
HG-U133B Annotations, CSV format, Release 25 (8 MB 03/18/08)
HG-U133B Annotations, MAGE-ML XML Format, Release 25 (31 MB 03/18/08)
HG-U133B BLASTP annotations, CSV Format, Release 25 (39 KB 03/18/08)
HG-U133B Orthologs/Homologs, CSV Format, Release 25 (2 MB 03/18/08)
HG-U133A Annotations, CSV format, Release 26 (11 MB 07/08/08)
HG-U133A Annotations, MAGE-ML XML Format, Release 26 (45 MB 07/08/08)
HG-U133A BLASTP annotations, CSV Format, Release 26 (6 KB 07/08/08)
HG-U133A Orthologs/Homologs, CSV Format, Release 26 (5 MB 07/08/08)
HG-U133B Annotations, CSV format, Release 26 (8 MB 07/08/08)
HG-U133B Annotations, MAGE-ML XML Format, Release 26 (31 MB 07/08/08)
HG-U133B BLASTP annotations, CSV Format, Release 26 (4 KB 07/08/08)
HG-U133B Orthologs/Homologs, CSV Format, Release 26 (2 MB 07/08/08)
HG-U133A Annotations, CSV format, Release 27 (11 MB 11/30/08)
HG-U133A Annotations, MAGE-ML XML Format, Release 27 (45 MB 11/24/08)
HG-U133A BLASTP annotations, CSV Format, Release 27 (27 KB 11/24/08)
HG-U133A Orthologs/Homologs, CSV Format, Release 27 (5 MB 11/24/08)
HG-U133B Annotations, CSV format, Release 27 (8 MB 11/30/08)
HG-U133B Annotations, MAGE-ML XML Format, Release 27 (32 MB 11/24/08)
HG-U133B BLASTP annotations, CSV Format, Release 27 (40 KB 11/24/08)
HG-U133B Orthologs/Homologs, CSV Format, Release 27 (3 MB 11/24/08)
HG-U133A Annotations, CSV format, Release 28 (11 MB 03/12/09)
HG-U133A Annotations, MAGE-ML XML Format, Release 28 (47 MB 03/12/09)
HG-U133A BLASTP annotations, CSV Format, Release 28 (3 KB 03/04/09)
HG-U133A Orthologs/Homologs, CSV Format, Release 28 (5 MB 03/12/09)
HG-U133B Annotations, CSV format, Release 28 (8 MB 03/12/09)
HG-U133B Annotations, MAGE-ML XML Format, Release 28 (32 MB 03/12/09)
HG-U133B BLASTP annotations, CSV Format, Release 28 (5 KB 03/04/09)
HG-U133B Orthologs/Homologs, CSV Format, Release 28 (3 MB 03/12/09)
HG-U133A Annotations, CSV format, Release 29 (12 MB 07/01/09)
HG-U133A Annotations, MAGE-ML XML Format, Release 29 (48 MB 07/01/09)
HG-U133A BLASTP annotations, CSV Format, Release 29 (3 KB 07/01/09)
HG-U133A Orthologs/Homologs, CSV Format, Release 29 (5 MB 07/01/09)
HG-U133B Annotations, CSV format, Release 29 (8 MB 07/01/09)
HG-U133B Annotations, MAGE-ML XML Format, Release 29 (33 MB 07/01/09)
HG-U133B BLASTP annotations, CSV Format, Release 29 (3 KB 07/01/09)
HG-U133B Orthologs/Homologs, CSV Format, Release 29 (3 MB 07/01/09)

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