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GeneChip WT cDNA Synthesis and Amplification Kit

The WT cDNA Synthesis and Amplification Kit supports the first-and second-cycle cDNA synthesis as well as the intermediate in vitro transcription amplification procedures.

Part # Description Unit Size Your Price(USD)
Qty
900672
GeneChip WT cDNA Synthesis and Amplification Kit
 sufficient for 10 reactions    Please Inquire
900673
GeneChip WT cDNA Synthesis and Amplification Kit
 sufficient for 30 reactions    Please Inquire
900652
GeneChip WT Sense Target Labeling and Control Reagents
 sufficient for 30 reactions    Please Inquire
     

Switch now to our Ambion® WT Expression Kit. Learn More >

GeneChip WT Sense Target Labeling and Control Reagents (900652) and GeneChip WT cDNA Synthesis and Amplification Kits (900672 and 900673) are being discontinued in June 2010.

 

The Whole Transcript (WT) Sense Target Labeling Assay Has an Updated Protocol.

The GeneChip® WT (Whole Transcript) cDNA Synthesis and Amplification Kit is optimized to be used in the first steps of the GeneChip® WT Sense Target (ST) Labeling Assay.

The WT cDNA Synthesis and Amplification Kit supports the first-and second-cycle cDNA synthesis as well as the intermediate in vitro transcription amplification procedures.

The input material is 1 µg of total RNA (1 µg recommended, with an acceptible range of 1-2 µg), which is treated with the RiboMinus™ Human/Mouse Transcriptome Isolation kit (from Invitrogen, not included in this kit) to remove the majority of the ribosomal RNA for enhanced sensitivity. The resulting product from the WT cDNA Synthesis and Amplification Kit is random-primed, single-stranded DNA in the sense orientation, ready to be fragmented and labeled with the GeneChip® WT Terminal Labeling Kit (available separately from Affymetrix).

An optional method may be used to reduce the starting material to 100 ng of total RNA (100 ng recommended, with an acceptible range of 100-300ng).

Sub-Kit 1: GeneChip® WT cDNA Synthesis Kit
For 10 Rxns
For 30 Rxns
T7-(N)6 Primers, 2.5 µg/µL 20 µL 60 µL
5X 1st Strand Buffer 100 µL 300 µL
DTT, 0.1M 50 µL 150 µL
dNTP, 10 mM 9 µL 27 µL
RNase Inhibitor 25 µL 75 µL
SuperScript™ II 90 µL 270 µL
MgCl2, 1M 40 µL 120 µL
DNA Polymerase I 6 µL 18 µL
RNase H 22 µL 66 µL
Random Primers, 3 µg/µL 20 µL 60 µL
dNTP+dUTP, 10 mM 20 µL 60 µL
RNase-free Water 4.44 mL 4.44 mL

Sub-Kit 2: GeneChip® WT cDNA Amplification Kit
For 10 Rxns
For 30 Rxns
10X IVT Buffer 50 µL 150 µL
IVT NTP Mix 200 µL 600 µL
IVT Enzyme Mix 50 µL 150 µL
IVT Control 10 µL 10 µL

The WT cDNA Synthesis and Amplification Kit is also available as a component of the GeneChip® WT Sense Target Labeling and Control Reagents Kit.

For research use only. Not for use in diagnostic procedures.

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