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GeneChip Mapping 500K Assay
GeneChip Mapping 500K Assay
The Affymetrix GeneChip® Mapping 500K Assay, in conjunction with the GeneChip® Mapping 500K Set, is designed to detect greater than 500,000 Single Nucleotide Polymorphisms (SNPs) in samples of genomic DNA.
Figure 1
The Affymetrix GeneChip® Mapping 500K Assay, in conjunction with the GeneChip® Mapping 500K Set, is designed to detect greater than 500,000 Single Nucleotide Polymorphisms (SNPs) in samples of genomic DNA. The Mapping 500K Set is comprised of two arrays and two assay kits. Each array and its corresponding assay kit are processed independently of the other array and assay kit. The protocol starts with 250 ng of genomic DNA per array and will generate SNP genotype calls for approximately 250,000 SNPs for each array of the two array set. The assay utilizes a strategy that reduces the complexity of human genomic DNA up to 10 fold by first digesting the genomic DNA with the Nsp I or Sty I restriction enzyme and then ligating sequences onto the DNA fragments. The complexity is further reduced by a PCR procedure optimized for fragments of a specified size range. Following these steps the PCR products (amplicons) are fragmented, end-labeled, and hybridized to a GeneChip array.
For more information, please review the GeneChip® Mapping 500K Assay Manual (pdf, 3.4 MB)
For research use only. Not for use in diagnostic procedures.
Application Notes
Microarrays in Cancer Research (pdf, 423 KB)
FAQs
Gene Expression Assay and Data Analysis
Manuals
Probe Set Data in MAGE-ML Format
Mapping 500K Assay Manual (pdf, 3.4 MB)
Package Inserts
Mapping 500K Assay Kit (pdf, 83 KB)
White Papers
BRLMM Algorithm for Affymetrix Mapping Arrays (pdf, 278 KB)
Required Products