GeneChip Human Genome U133 Plus 2.0 Array
The GeneChip® Human Genome U133 Plus 2.0 Array is first and most comprehensive whole human genome expression array.
First and most comprehensive whole human genome expression array
- Convenient one-array view.
- Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts.
- Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform.
- GeneChip® System: Richer data, sharper insights, better decisions
Array Profile
All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. The sequences from which these probe sets were derived were selected from GenBank®, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).
In addition, there are 9,921 new probe sets representing approximately 6,500 new genes. These gene sequences were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from the UniGene database (Build 159, January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31).
For research use only. Not for use in diagnostic procedures.

Application Notes
Microarrays in Cancer Research (pdf, 423 KB)
Microarray Applications in Infectious Disease (pdf, 252 KB)
Comparison Sheets / Array Comparisons
Canine 2.0 to HG-U133 Plus, Good Match (zip, 182 KB)
HG-U95 to HG-U133 Plus, Best Match (zip, 35 KB)
Bovine to HG-U133 Plus, Best Match (zip, 100 KB)
Bovine to HG-U133 Plus, Complex (zip, 598 KB)
Bovine to HG-U133 Plus, No Match (zip, 27 KB)
HG-U95 to HG-U133 Plus, No Match (zip, 1 KB)
Rat 230 to HG-U133 Plus, Good Match (zip, 1 KB)
Bovine to HG-U133 Plus, Good Match (zip, 103 KB)
Canine to HG-U133 Plus, All (zip, 433 KB)
HG-U133 to HG-U133 Plus, Complex (zip, 24 MB)
Canine 2.0 to HG-U133 Plus, Complex Match (zip, 1.4 MB)
HG-U133 Plus 2.0 to Rhesus Good Match
Canine 2.0 to HG-U133 Plus, Best Match (zip, 164 KB)
HG-U133 Plus to Human Gene 1.0 ST, Best Match (zip, 651 KB)
HG-U133 to HG-U133 Plus, Good Match (zip, 5 KB)
HG-U133 Plus to Human Gene 1.0 ST, Complex Match (zip, 2.7 MB)
Porcine to HG-U133 Plus, No Match (zip, 28 KB)
Canine to HG-U133 Plus, Best Match (zip, 19 KB)
HG-U95 to HG-U133 Plus, Complex (zip, 37 MB)
HG-U133 Plus 2.0 to Rhesus Complex
HG-U133 to HG-U133 Plus, Best Match (zip, 5 KB)
HG-U133 Plus to Human Exon 1.0 ST, Complex Match (zip, 36 MB)
HG-U133 Plus 2.0 to Rhesus No Match
HG-U133 Plus to Human Exon 1.0 ST, Good Match (zip, 937 KB)
HG-U133 Plus 2.0 to Rhesus Best Match
Rat 230 to HG-U133 Plus, Best Match (zip, 1 KB)
HG-U133 Plus to Human Exon 1.0 ST, No Match (zip, 1.5 KB)
Rat 230 to HG-U133 Plus, No Match (zip, 79 KB)
HG-U133 Plus to Human Exon 1.0 ST, Best Match (zip, 899 KB)
Canine 2.0 to HG-U133 Plus, No Match (zip, 32 KB)
HG-U133 Plus to Human Gene 1.0 ST, Good Match (zip, 658 KB)
HG-U133 Plus to Human Gene 1.0 ST, No Match (zip, 38 KB)
Porcine to HG-U133 Plus, Good Match (zip, 112 KB)
Canine to HG-U133 Plus, Good Match (zip, 20 KB)
Porcine to HG-U133 Plus, Complex (zip, 596 KB)
Canine to HG-U133 Plus, No Match (zip, 30 KB)
HG-U133 to HG-U133 Plus, No Match (zip, 1 KB)
HG-U95 to HG-U133 Plus, Good Match (zip, 37 KB)
Porcine to HG-U133 Plus, Best Match (zip, 109 KB)
Rat 230 to HG-U133 Plus, Complex (zip, 225 KB)
Data Sheets
Human Genome U133 Arrays (pdf, 1.76 MB)
FAQs
Library Files
Human Genome U133 Plus 2.0 Array (zip, 30 MB)
Manuals
Probe Set Data in MAGE-ML Format
3' IVT Express Kit User Manual (pdf, 1.6 MB)
HT 3' IVT Express Kit (pdf 5.93 MB)
Package Inserts
Human Genome U133 Plus 2.0 Array (pdf, 493 KB)
Technical Notes
GeneChip Expression Platform: Comparison, Evolution, and Performance (pdf, 269 KB)
Other
MicroArrayQuality Control Study (MAQC - pdf, 1.5 MB)
NetAffx Alignment Files
HG-U133_Plus_2 Alignments to Genome, PSL (8 MB 09/28/06)
NetAffx Annotation Files
HG-U133_Plus_2 Annotations, CSV format, Release 30 (24 MB 11/15/09)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 30 (97 MB 11/15/09)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 30 (48 KB 11/16/09)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 30 (9 MB 11/15/09)
Sequence Files
HG-U133_Plus_2 Consensus Sequences, FASTA (24 MB 08/20/08)
HG-U133_Plus_2 Control Sequences, FASTA (20 KB 10/17/03)
HG-U133_Plus_2 Exemplar Sequences, FASTA (13 MB 10/17/03)
HG-U133_Plus_2 Probe Sequences, FASTA (12 MB 08/20/08)
HG-U133_Plus_2 Probe Sequences, Tabular (10 MB 08/20/08)
HG-U133_Plus_2 Target Sequences, FASTA (12 MB 08/20/08)
Archived NetAffx Annotation Files
HG-U133_Plus_2 Annotations, MAGE-ML XML, Release 20 (95 MB 06/15/07)
HG-U133_Plus_2 Annotations, CSV, Release 20 (21 MB 05/30/07)
HG-U133_Plus_2 Annotations, BLASTX, Release 20 (2 MB 06/23/04)
HG-U133_Plus_2 Annotations, BLASTP, Release 20 (5 MB 07/14/06)
HG-U133_Plus_2 Annotations, Orthologs, Release 20 (8 MB 08/21/06)
HG-U133_Plus_2 Annotations, CSV format, Release 21 (21 MB 05/31/07)
HG-U133_Plus_2 Annotations, MAGE-ML XML format, Release 21 (97 MB 06/15/07)
HG-U133_Plus_2 BLASTP annotations, CSV format, Release 21 (5 MB 11/15/06)
HG-U133_Plus_2 Orthologs/Homologs, CSV format, Release 21 (8 MB 11/30/06)
HG-U133_Plus_2 Annotations, CSV format, Release 22 (22 MB 05/31/07)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 22 (90 MB 06/15/07)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 22 (148 KB 03/09/07)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 22 (9 MB 03/09/07)
HG-U133_Plus_2 Annotations, CSV format, Release 23 (20 MB 07/12/07)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 23 (86 MB 07/12/07)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 23 (149 KB 07/12/07)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 23 (8 MB 07/12/07)
HG-U133_Plus_2 Annotations, CSV format, Release 24 (20 MB 11/06/07)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 24 (86 MB 11/06/07)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 24 (8 KB 11/06/07)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 24 (7 MB 11/06/07)
HG-U133_Plus_2 Annotations, CSV format, Release 25 (21 MB 03/18/08)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 25 (87 MB 03/18/08)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 25 (68 KB 03/18/08)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 25 (7 MB 03/18/08)
HG-U133_Plus_2 Annotations, CSV format, Release 26 (22 MB 07/08/08)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 26 (90 MB 07/09/08)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 26 (12 KB 07/08/08)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 26 (8 MB 07/08/08)
HG-U133_Plus_2 Annotations, CSV format, Release 27 (22 MB 11/30/08)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 27 (97 MB 11/24/08)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 27 (79 KB 11/24/08)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 27 (9 MB 11/24/08)
HG-U133_Plus_2 Annotations, CSV format, Release 28 (23 MB 03/12/09)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 28 (94 MB 03/12/09)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 28 (9 KB 03/04/09)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 28 (9 MB 03/12/09)
HG-U133_Plus_2 Annotations, CSV format, Release 29 (23 MB 07/01/09)
HG-U133_Plus_2 Annotations, MAGE-ML XML Format, Release 29 (95 MB 07/01/09)
HG-U133_Plus_2 BLASTP annotations, CSV Format, Release 29 (8 KB 07/01/09)
HG-U133_Plus_2 Orthologs/Homologs, CSV Format, Release 29 (9 MB 07/01/09)

Required Products
Related Products
| GeneChip HT HG-U133+ PM Array Plate |
| Affymetrix® Human Genome U219 Array Plate |
| GeneChip Human Gene 1.0 ST Array |
| GeneChip Human Exon 1.0 ST Array |
| Human Gene 1.1 ST Array Plate |



