GeneChip Human Exon 1.0 ST Array
With approximately four probes per exon and roughly 40 probes per gene, the GeneChip® Human Exon 1.0 ST Array enables two complimentary levels of analysis - gene expression and alternative splicing.
With approximately four probes per exon and roughly 40 probes per gene, the GeneChip® Human Exon 1.0 ST Array enables two complementary levels of analysis—gene expression and alternative splicing.
Multiple probes per exon enable "exon-level" analysis and allow you to distinguish between different isoforms of a gene. This exon-level analysis on a whole-genome scale opens the door to detecting specific alterations in exon usage that may play a central role in disease mechanism and etiology.
The second level is "gene-level" expression analysis, in which multiple probes on different exons are summarized into an expression value of all transcripts from the same gene.
Exon arrays provide the most comprehensive coverage of the genome, including empirically supported and predicted transcribed sequences, enabling the discovery of previously unidentified novel events.
Features of this integrated solution include:
- Highest resolution expression analysis, interrogating over 1 million exon clusters within the known and predicted transcribed regions of the entire genome. Read more >
- Tailored GeneChip® Whole Transcript (WT) Assay and Reagents utilizing a random priming method for generating sense targets along the entire length of the transcripts.
- Flexible data analysis solutions and comprehensive annotation, giving you the opportunity to explore and dissect the data in various user-defined workflows.
Learn More
Explore the Exon Array Design
GeneChip® Exon Array Sample Data
Technical Note, Array Design for the Human Exon 1.0 ST Array (pdf, 348 KB)
Technical Note, Identifying and Validating Alternative Splicing Events
New XRAY demos for exon expression analysis
View a two-minute demo on a typical workflow using Biotique's XRAY for the analysis of GeneChip® Exon Arrays
Windows Media Player | Quicktime
View a two-minute demo on the interpretation of GeneChip® Exon Array data from Biotique's XRAY
Windows Media Player | Quicktime
| Design Statistics Summary | |
|---|---|
| Probe Sets | 1.4 million |
| Exon Clusters | > 1 million |
| Supported by Putative Full-Length mRNA | 289,961 probe sets |
| Supported by Ensembl Transcripts | 306,583 probe sets |
| Supported by EST | 665,175 probe sets |
| Supported by Mouse or Rat mRNA | 220,262 probe sets |
| Supported by Gene Prediction | 883,105 probe sets |
| Probe Selection Region | Along the entire length of the transcripts |
| Probes/Probe Selection Region | 4¹ |
| Background Subtraction Strategy | Median intensity of up to 1,000 background probes with the same GC content |
| Total Features Per Array | >5,500,000 |
| Interrogated Strand | Sense² |
¹ About 10 percent of the exon probe sets have fewer than four probes due to the probe selection region length and sequence constraints.
²The probes tiled on the array are designed in the anti-sense orientation, requiring sense-strand labeled targets to be hybridized to the array. By convention, the array is called ST array representing the necessity of using sense targets (the labeled samples to be hybridized to the array).
Human Exon 1.0 ST Array Starter Pack
The GeneChip® Human Exon 1.0 ST Array provides the highest resolution expression analysis, interrogating over 1 million exon clusters within the known and predicted transcribed regions of the entire genome. For optimal results with the GeneChip® Exon Array system, it is highly recommended that new users begin with the GeneChip® Human Exon 1.0 ST Array Starter Pack.
*Each Starter Pack contains:
- GeneChip® Human Exon 1.0 ST Array, 30 arrays
- GeneChip® WT Sense Target Labeling and Control Reagents, 30 reactions
- On-site training (scheduled as needed) on using the GeneChip® Exon Array system
The Starter Pack includes on-site training with the following arrays and reagents:
- GeneChip® Human Exon 1.0 ST Array, 10 pack
- GeneChip® WT cDNA Synthesis and Amplification Kit, 10 reactions
- GeneChip® WT Terminal Labeling Kit, 10 reactions
- GeneChip® Sample Cleanup Module, 30 reactions
- GeneChip® Eukaryotic Poly-A RNA Control Kit, 100 reactions
- GeneChip® Hybridization Control Kit, 30 reactions
For research use only. Not for use in diagnostic procedures.

Application Notes
Whole-transcript Expression Analysis (pdf, 557 kb)
Comparison Sheets / Array Comparisons
Comparison Spreadsheets User's Guide (pdf, 82 KB)
Human Exon 1.0 ST to Human Gene 1.0 ST, No Match (zip 500 B)
Human Exon 1.0 ST to Human Gene 1.0 ST, Good Match (zip, 521 KB)
Human Exon 1.0 ST to Human Gene 1.0 ST, Best Match (zip, 485 KB)
HG-U133 Plus to Human Exon 1.0 ST, Best Match (zip, 899 KB)
HG-U133 Plus to Human Exon 1.0 ST, No Match (zip, 1.5 KB)
HG-U133 Plus to Human Exon 1.0 ST, Good Match (zip, 937 KB)
HG-U133 Plus to Human Exon 1.0 ST, Complex Match (zip, 36 MB)
Human Exon 1.0 ST to Human Gene 1.0 ST, Complex Match (zip, 29 MB)
Data Sheets
Exon Array System (pdf, 416 KB)
FAQs
Library Files
Human Exon 1.0 ST Array, GCOS (zip, 2.2 MB)
Unsupported Human Exon 1.0 ST Array CDF Files (zip, 205 MB)
Human Exon 1.0 ST Array Analysis (zip, 131 MB)
Manuals
Whole Transcript (WT) Sense Target Labeling Assay (pdf, 1.18 MB)
Package Inserts
Human Exon 1.0 ST Array (pdf, 80 KB)
Publications
Publications and Reference for Affymetrix GeneChip Exon and Gene 1.0 ST Arrays (pdf, 74 KB)
Quick Reference Cards
QC Metrics for Exon and Gene Design Expression Arrays (pdf, 123 KB)
Sample Data
Technical Notes
Array Design for the Human Exon 1.0 ST Array (pdf, 348 KB)
Guide to Probe Logarithmic Intensity Error (PLIER) Estimation (pdf, 294 KB)
White Papers
Quality Assessment of Exon and Gene 1.0 ST Arrays (pdf, 180 KB)
Gene Signal Estimates from Exon Arrays v1.0 (pdf, 428 KB)
Exon Related Changes FAQ (pdf, 268 KB)
Exon Probeset Annotations and Transcript Cluster Groupings v1.0 (pdf, 87 KB)
Alternative Transcript Analysis Methods for Exon Arrays v1.1 (pdf, 79 KB)
Exon Array Background Correction v1.0 (pdf, 78 KB)
Other
Design Time Annotation Files
HuEx-1_0-st-v2 Annotations, Probeset, Hg18/Build36, CSV (43 MB 10/06/06)
HuEx-1_0-st-v2 Annotations, Probeset, Hg17/Build35, CSV (43 MB 10/06/06)
HuEx-1_0-st-v2 Annotations, Probeset, Hg16/Build34, CSV (43 MB 10/06/06)
HuEx-1_0-st-v2 Mappings, TSV (3 MB 10/11/05)
HuEx-1_0-st-v2 Annotations, Full, Hg18/Build 36, GFF (339 MB 06/20/06)
HuEx-1_0-st-v2 Annotations, Full, Hg17/Build 35, GFF (340 MB 01/13/06)
HuEx-1_0-st-v2 Transcript Cluster ID Mappings to HG-U133 Plus 2.0 (415 KB 08/28/06)
HuEx-1_0-st-v2 Annotations, Full, Hg16/Build 34, GFF (340 MB 01/13/06)
IGB Annotation Files
HuEx-1_0-st-v2 Annotations, Hg16/Build 34, BGN (48 MB 02/09/06)
HuEx-1_0-st-v2 Annotations, Hg18/Build 36, BGN (47 MB 07/14/06)
HuEx-1_0-st-v2 Annotations, Hg17/Build 35, BGN (47 MB 02/09/06)
NetAffx Annotation Files
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 30 (27 MB 11/09/09)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 30 (42 MB 11/09/09)
HuEx-1_0-st-v2 Annotations CSV README, Release 30 (39 KB 11/09/09)
Sequence Files
HuEx-1_0-st-v2 Transcript Cluster Sequences, FASTA format (104 MB 03/19/08)
HuEx-1_0-st-v2 Sequence Files README (12 KB 03/19/08)
HuEx-1_0-st-v2 Probe Sequences, FASTA format (177 MB 03/19/08)
HuEx-1_0-st-v2 Probeset Sequences FASTA Format (89 MB 03/19/08)
HuEx-1_0-st-v2 Sequence Files README (12 KB 03/19/08)
HuEx-1_0-st-v2 Probe Sequences, tabular format (150 MB 03/19/08)
Archived NetAffx Annotation Files
HuEx-1_0-st-v2 Annotations CSV README, Release 24 (37 KB 11/12/07)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 24 (39 MB 11/12/07)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 24 (16 MB 11/12/07)
HuEx-1_0-st-v2 Annotations CSV README, Release 25 (37 KB 03/19/08)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 25 (39 MB 03/20/08)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 23 (20 MB 09/14/07)
HuEx-1_0-st-v2 Annotations CSV README, Release 26 (39 KB 07/08/08)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 22 (43 MB 03/28/07)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 26 (39 MB 07/17/08)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 26 (19 MB 07/17/08)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 25 (16 MB 03/20/08)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 23 (43 MB 09/14/07)
HuEx-1_0-st-v2 Annotations CSV README, Release 23 (37 KB 07/13/07)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 22 (23 MB 06/11/07)
HuEx-1_0-st-v2 Annotations CSV README, Release 22 (48 KB 03/28/07)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 21 (22 MB 06/11/07)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 21 (44 MB 11/27/06)
HuEx-1_0-st-v2 Annotations CSV README, Release 21 (36 KB 11/27/06)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 20 (21 MB 07/20/06)
HuEx-1_0-st-v2 Annotations CSV README, Release 27 (39 KB 11/24/08)
HuEx-1_0-st-v2 Probeset Annotations, CSV, Release 20 (44 MB 07/20/06)
HuEx-1_0-st-v2 Annotations README (33 KB 07/20/06)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 27 (40 MB 11/24/08)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 29 (23 MB 07/07/09)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 29 (41 MB 07/23/09)
HuEx-1_0-st-v2 Annotations CSV README, Release 29 (39 KB 06/24/09)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 28 (20 MB 03/11/09)
HuEx-1_0-st-v2 Probeset Annotations, CSV Format, Release 28 (40 MB 03/11/09)
HuEx-1_0-st-v2 Annotations CSV README, Release 28 (39 KB 03/11/09)
HuEx-1_0-st-v2 Transcript Cluster Annotations, CSV, Release 27 (19 MB 11/24/08)
Archived Sequence Files
HuEx-1_0-st-v2 Probe Sequences, FASTA format (162 MB 11/17/06)
HuEx-1_0-st-v2 Probe Sequences, tabular format (149 MB 07/13/07)
HuEx-1_0-st-v2 Probe Sequences, FASTA format (162 MB 07/13/07)
HuEx-1_0-st-v2 Transcript Cluster Sequences, FASTA format (103 MB 05/10/07)
HuEx-1_0-st-v2 Probe Sequences, tabular format (149 MB 11/17/06)
HuEx-1_0-st-v2 Sequence Files README (11 KB 05/10/07)
HuEx-1_0-st-v2 Probe Sequences, Tabular (148 MB 01/16/06)
HuEx-1_0-st-v2 Probeset Sequences FASTA Format (88 MB 03/20/07)
HuEx-1_0-st-v2 Probe Sequences, FASTA (158 MB 01/16/06)
HuEx-1_0-st-v2 Sequence Files README (11 KB 07/13/07)

Required Products
Related Products
| GeneChip Human Gene 1.0 ST Array |
| Human Gene 1.1 ST Array Plate |



