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GeneChip HT 3' IVT Express Kit
GeneChip HT 3' IVT Express Kit
The GeneChip® HT 3' IVT Express Kit is available in 24-reaction and 96-reaction sizes.
The GeneChip® HT 3' IVT Express Kit is the latest and most effective option for target preparation on Affymetrix' industry-standard 3' expression arrays on the Beckman Biomek® FXp Target Prep Express Instrument.
HT 3' IVT Express Kit
The kits feature:
- Low RNA input requirements - as little as 100 ng total RNA for a single round of amplification
- Streamlined workflow - no initial primer annealing or cDNA purification step
- The option to decrease target labeling time with appropriate inputs of total RNA
- Fewer reagents needed for entire amplification and labeling process
- A simple protocol for ease of use, convenience, and a high rate of success
- Magnetic bead purification for enhanced recovery
- A complete kit that includes poly-A RNA and hybridization controls
With enhanced performance, fewer required reagents, and a simple protocol, target preparation has never been so easy.
Figure 1
The GeneChip HT 3' IVT Express Kit includes:
- All reagents needed to generate and label aRNA target
- Poly-A RNA controls and hybridization controls
- Magnetic bead purification for enhanced recovery
About controls
Poly-A controls (lys, phe, thr and dap) monitor the target labeling process from start to finish. They serve as sensitivity indicators of target prep and labeling efficiency. This kit contains four exogenous, pre-mixed, poly-adenylated prokaryotic controls that are spiked directly into RNA samples before target labeling. Their resultant signal intensities on GeneChip Arrays serve as indicators of the labeling reaction efficiency.
The 20X Eukaryotic Hybridization Controls are comprised of a mixture of biotinylated and fragmented cRNA of bioB, bioC, bioD, and cre in staggered concentrations, ready to be added directly to the hybridization cocktail. These controls facilitate monitoring of the hybridization process for troubleshooting. Control Oligo B2 is included to provide alignment signals for image analysis.
For research use only. Not for use in diagnostic procedures.
Manuals
Site Prep Guide, HT 3' IVT Express on Biomek FXP Target Prep Express (pdf, 411 KB)
HT 3' IVT Express Kit (pdf 8.41 MB)
Package Inserts
Affymetrix Labware Kit for 3' IVT Method on Biomek FXP Target Prep Express (pdf, 849 KB)
HT 3' IVT Express Kit (pdf, 182 KB)
Safety Data Sheets
GeneChip HT 3' IVT Express Kit (pdf, 689 KB)
White Papers
HT 3' IVT Express Kit (pdf 8.41 MB)