GeneChip HT HG-U133+ PM Array Plate
The GeneChip® HT HG-U133+ PM Array Plate enables high-throughput expression profiling of 16, 24, or 96 samples at a time.
Proven performance from the industry standard
The GeneChip® HT HG-U133+ PM Array Plate enables high-throughput expression profiling of 16, 24, or 96 samples at a time using the same content as the industry-standard GeneChip® Human Genome U133 Plus 2.0 Array*.
The HT HG-U133+ PM Array Plate enables you to:
- Measure gene expression of more than 47,000 transcripts and variants, including more than 33,000 well-characterized genes and UniGene clusters per sample
- Get accurate and reproducible data by using multiple independent measurements for each transcript
Scatter plots represent median MAQC B - MAQC A signals for each array type. The high correlation coefficients indicate similar biology is being observed.
Key Benefits
- Enables increased productivity and efficiency through parallel processing
- Process 16, 24, or 96 samples on a single array plate
- Same industry-leading performance and content as the Human Genome U133 Plus 2.0 Array in cartridge format
- Strong signal and fold change correlation to previous plate and cartridge designs
- Multiple independent measurements per transcript for increased confidence in your results
- Interrogates more sequence than a single probe measurement
- Conveniently packaged
- Individual 16-, 24-, and 96-array plates available
- All processing trays included
Content Profile
Sequences used in the design of the arrays were selected from GenBank®, dbEST and RefSeq. The majority of sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).
An additional set of sequence clusters were created from Build 159 of UniGene (January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31). Sequences were further analyzed for correct orientation, false priming, false clustering, alternative splicing and alternative polyadenylation.
* Two design changes were introduced with the GeneChip HT HG-U133+ PM Array Plate:
- Only perfect match (PM) probes from the cartridge design were retained while mismatch (MM) probes were removed.
- Empirical data was used to select the best-performing probes resulting in reducing the number of PM probes
- 42,461 probe sets were reduced from 11 to nine probes
- Six probe sets were reduced from 11 to 10
For research use only. Not for use in diagnostic procedures.

Data Sheets
HT PM Array Plate System for Human, Mouse, and Rat (pdf, 448 KB)
HT Array Replacement Guideline (pdf, 179 KB)
FAQs
Library Files
AGCC 3.x HT HG-U133PM Plus Library Files (zip, 13.9 MB)
Manuals
GeneTitan® Instrument User's Guide for Expression Array Plates (pdf, 3.33 MB)
Quick Reference Cards
QC Metrics for Human, Mouse and Rat HT PM Array Plates (pdf, 111 KB)
Sample Data
Human Tissue Panel (zip, 216 MB)
Technical Notes
Performance and Validation of GeneChip HT Human, Mouse and Rat PM Array Plates (pdf, 678 KB)
White Papers
HT and Cartridge IVT Expression Arrays (pdf, 601 KB)
Other
Installing GeneTitan Scripts for 16 peg 3' IVT HT Array Plates (zip, 41 KB)
NetAffx Annotation Files
HT_HG-U133_Plus_PM BLASTP annotations, CSV Format, Release 30 (49 KB 11/16/09)
HT_HG-U133_Plus_PM Annotations, MAGE-ML XML Format, Release 30 (77 MB 11/15/09)
HT_HG-U133_Plus_PM Annotations, CSV format, Release 30 (24 MB 11/15/09)
Sequence Files
HT_HG-U133_Plus_PM Probe Sequences, FASTA format (10 MB 03/11/09)
HT_HG-U133_Plus_PM Probe Sequences, tabular format (9 MB 03/11/09)
HT_HG-U133_Plus_PM Target Sequences FASTA Format (12 MB 01/06/09)
HT_HG-U133_Plus_PM Consensus Sequences FASTA Format (35 MB 01/06/09)
Archived Library Files
HT_HG-U133_Plus_PM Report Controls (1 KB 08/12/08)
Archived NetAffx Annotation Files
HT_HG-U133_Plus_PM BLASTP annotations, CSV Format, Release 28 (9 KB 03/04/09)
HT_HG-U133_Plus_PM Annotations, CSV format, Release 27 (22 MB 02/26/09)
HT_HG-U133_Plus_PM Annotations, CSV format, Release 28 (23 MB 03/12/09)
HT_HG-U133_Plus_PM Annotations, MAGE-ML XML Format, Release 28 (76 MB 03/12/09)
HT_HG-U133_Plus_PM Annotations, CSV format, Release 29 (23 MB 07/01/09)
HT_HG-U133_Plus_PM Annotations, MAGE-ML XML Format, Release 29 (76 MB 07/01/09)
HT_HG-U133_Plus_PM BLASTP annotations, CSV Format, Release 29 (8 KB 07/01/09)
HT_HG-U133_Plus_PM Annotations, CSV format, Release 27 (22 MB 11/30/08)
Archived Sequence Files
HT_HG-U133_Plus_PM Probe Sequences, FASTA format (10 MB 07/16/08)
HT_HG-U133_Plus_PM Probe Sequences, tabular format (9 MB 07/16/08)

Required Products
| GeneChip HT 3' IVT Express Kit |
| GeneTitan® Hybridization, Wash, and Stain Kits |
| Affymetrix Expression Console Software |
| GeneChip Command Console Software (AGCC) |
| GeneTitan family of instruments |
Related Products
| Human Gene 1.1 ST Array Plate |
| GeneChip Human Genome U133 Plus 2.0 Array |
| Affymetrix® Human Genome U219 Array Plate |






