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GeneChip 3' IVT Express Kit
GeneChip 3' IVT Express Kit
The GeneChip® 3' IVT Express Kit is the latest and most effective option for target preparation on Affymetrix' industry-standard 3' expression arrays.
The GeneChip® 3' IVT Express Kit is the latest and most effective option for target preparation on Affymetrix' industry-standard 3' expression arrays.
3' IVT Express Kit
The kits feature:
- Low RNA input requirements - as little as 50 ng total RNA for a single round of amplification
- Streamlined workflow - no initial primer annealing or cDNA purification step, and a 1.25-hour second-strand cDNA synthesis time
- The option to decrease target labeling time to a single day with appropriate inputs of total RNA
- Fewer reagents needed for entire amplification and labeling process
- A simple protocol for ease of use, convenience, and a high rate of success
- Magnetic bead purification for enhanced recovery
- A complete kit that includes poly-A RNA and hybridization controls
With enhanced performance, fewer required reagents, and a simple protocol, target preparation has never been so easy.
Figure 1
The GeneChip 3' IVT Express Kit includes:
- All reagents needed to generate and label aRNA target
- Poly-A RNA controls and hybridization controls
- Magnetic bead purification for enhanced recovery
- Plastic consumables needed to run the assay
The GeneChip 3' IVT Express Kit is available in 10-reaction and 30-reaction sizes.
The kit can be purchased alone or bundled with popular 3' arrays for additional savings:
- GeneChip® Human Genome U133 Plus 2.0 Arrays (10 and 30 packs)
- GeneChip® Mouse Genome 430 2.0 Arrays (10 and 30 packs)
- GeneChip® Rat Genome 230 2.0 Arrays (10 and 30 packs)
Additional products needed to run the complete Affymetrix solution include the GeneChip® Hybridization, Wash, and Stain Kit.
About Poly-A controls
Poly-A controls (lys, phe, thr and dap) monitor the target labeling process from start to finish. They serve as sensitivity indicators of target prep and labeling efficiency. This kit contains four exogenous, pre-mixed, poly-adenylated prokaryotic controls that are spiked directly into RNA samples before target labeling. Their resultant signal intensities on GeneChip Arrays serve as indicators of the labeling reaction efficiency.
The 20X Eukaryotic Hybridization Controls are comprised of a mixture of biotinylated and fragmented cRNA of bioB, bioC, bioD, and cre in staggered concentrations, ready to be added directly to the hybridization cocktail. These controls facilitate monitoring of the hybridization process for troubleshooting. Control Oligo B2 is included to provide alignment signals for image analysis.
For research use only. Not for use in diagnostic procedures.
Data Sheets
3' IVT Express Kit (pdf, 278 KB)
FAQs
Manuals
Expression Wash, Stain and Scan User Manual for Cartridge Arrays (pdf, 5.41 MB)
3' IVT Express Kit User Manual (pdf, 1.6 MB)
Package Inserts
3' IVT Express Kit (pdf, 566 KB)
Quick Reference Cards
3' IVT Express Kit Assay (pdf, 631 KB)
Safety Data Sheets
3' IVT Express Kit (zip, 1.3 MB)
Sample Data
3' IVT Express Kit (zip, 150 MB)
Technical Notes
3' IVT Express and One-Cycle Comparison (pdf, 245 KB)
White Papers
3' IVT Express Kit (pdf, 740 KB)
Required Products