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Prokaryotic Target Labeling Assay

Prokaryotic Target Labeling Assay

Part # Description Unit Size Your Price (USD)
Qty
900301
Control Oligo B2, 3nM
Sufficient for 30 reactions    Please Inquire
900542
GeneChip DNA Labeling Reagent, 7.5 mM
sufficient for 30 reactions    Please Inquire
900433
GeneChip Poly-A RNA Control Kit
sufficient for approx. 100 reactions    Please Inquire
900720
GeneChip Hybridization, Wash, and Stain Kit
sufficient for 30 reactions    Please Inquire
900721
Wash Buffer A
800 mL    Please Inquire
900722
Wash Buffer B
600 mL    Please Inquire
     

The Affymetrix Prokaryotic Target Labeling Assay is used for 3' expression studies with Affymetrix prokaryotic GeneChip® Brand Arrays. The assay utilizes reverse transcriptase and random hexamer primers to produce DNA complementary to the RNA. The cDNA products are then fragmented by DNase I and labeled with terminal transferase and biotinylated GeneChip® DNA Labeling Reagent at the 3' termini. Functionally tested on GeneChip® Brand Arrays, Affymetrix recommends the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.

Components Include:

For research use only. Not for use in diagnostic procedures.

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